Muhammad Israr

3 Questions 5 Answers 0 Followers

Questions related from Muhammad Israr

I need Enzyme Assay Protocol for D-Amino Acid Transferase, please let me know if anyone worked on DAAT?

Hi all, I am going to engineer D-Amino Acid Transferase from bacterial source for which I need Enzyme Assay Protocol to determine the specific activity of wild type and mutant DAATs. Please if...

05 June 2024 2,680 3 View

What is the best method to find the accurate optimum temperatre and pH and also find the thermal stability of wild type enzyme and its mutants ?

Hi ! I am finding the optimum temperature and pH of thermostable enzyme and its mutants. I find the optimum temperature of wild type enzyme and its mutants as 80C and 70C at 10 minutes...

12 December 2017 4,858 1 View

What concentration of DNA template should be used for best result of PCR for site directed mutagenesis ?

Hi I used DNA template having concentration of 31ng for my mutation PCR, but no mutation occur after sequencing. my PCR protocol is as...

13 December 2016 2,070 6 View