Muhammad Israr

2 Questions 4 Answers 0 Followers

Questions related from Muhammad Israr

What is the best method to find the accurate optimum temperatre and pH and also find the thermal stability of wild type enzyme and its mutants ?

Hi ! I am finding the optimum temperature and pH of thermostable enzyme and its mutants. I find the optimum temperature of wild type enzyme and its mutants as 80C and 70C at 10 minutes...

12 December 2017 4,832 1 View

What concentration of DNA template should be used for best result of PCR for site directed mutagenesis ?

Hi I used DNA template having concentration of 31ng for my mutation PCR, but no mutation occur after sequencing. my PCR protocol is as...

13 December 2016 2,056 6 View