I want to extract glycosides from my mangrove leaf samples, but my laboratory lacks an incubator capable of reaching temperatures above 60°C. The paper I am following specifies a minimum temperature of 75°C for effective glycoside bond cleavage, which aligns with the literature indicating that glycoside bonds require temperatures between 70°C and 100°C for cleavage, while avoiding thermal degradation of certain compounds. Given my equipment limitations, how can I effectively extract these glycosidic compounds from my mangrove leaves? Additionally, I am confused about the significant time difference in two papers: one suggests 30 minutes at 85°C, while the other recommends 4 hours at 75°C. How do researchers typically approach and interpret such differences in time and temperature for glycoside extraction? Your valuable feedback and suggestions would be greatly appreciated.
- Badges
- Science topic
- Similar topics
- Mathematics
- Statistics
- Sampling Design
- Sample Size