Do murine isolated NK cells in culture need to be trypsinized for passage or are they floating?

09 October 2016 6,032 4 View

How to detect growth changes by addition of conditioned media?

02 March 2016 5,155 1 View

How best to dilute camptothecin to i.p. inject 50 mg/kg into mouse?

02 March 2016 9,253 2 View

Can you use PE Annexin v apoptosis detection kit i with mouse lung tissue?

01 February 2016 9,233 1 View

Can someone help me calculate drug dilutions and dosing for i.p. injection?

I have an undiluted drug in powder form. I need to give it to my 20 gram mouse at a dose of 10 mg/Kg. The drug is docetaxel and its MW is 807.87922. The drug product sheet says that it is soluble...

06 July 2015 4,946 3 View

Can someone help me with genotyping PCR?

06 July 2015 1,932 12 View

Was I correct in using cDNA to check for mutant vs. wildtype p53?

07 August 2014 815 16 View

Does anyone have experience with an efficient assay for measuring sister chromatid exchange in cell culture? Any good commercial kits available?

I would appreciate an efficient protocol for measuring sister chromatid exchange or suggestions for a commercial kit available to purchase.

07 August 2014 7,598 3 View

How does one prepare 300 uM hydrogen peroxide from 3 wt % H2O2 solution ?

Confused trying to change weight % into molarity using sigma's hydrogen peroxide.

06 July 2014 4,953 4 View

Has anyone used a protease to chop up a large protein like BRCA2 in order to detect by WB?

Having trouble detecting BRCA2 protein by Western Blot. I used a 3-8% Tris-Acetate gel, 40 ug of protein and ran the gel for 4 hours. To transfer I ran the wet apparatus at 4C overnight at 25...

05 June 2014 443 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View