Do murine isolated NK cells in culture need to be trypsinized for passage or are they floating?

09 October 2016 6,032 4 View

How to detect growth changes by addition of conditioned media?

02 March 2016 5,155 1 View

Can you use PE Annexin v apoptosis detection kit i with mouse lung tissue?

01 February 2016 9,233 1 View

Can someone help me calculate drug dilutions and dosing for i.p. injection?

I have an undiluted drug in powder form. I need to give it to my 20 gram mouse at a dose of 10 mg/Kg. The drug is docetaxel and its MW is 807.87922. The drug product sheet says that it is soluble...

06 July 2015 4,946 3 View

Can someone help me with genotyping PCR?

06 July 2015 1,932 12 View

What is the best method for transfection with RSV promoter?

07 August 2014 9,442 2 View

Was I correct in using cDNA to check for mutant vs. wildtype p53?

07 August 2014 815 16 View

Does anyone have experience with an efficient assay for measuring sister chromatid exchange in cell culture? Any good commercial kits available?

I would appreciate an efficient protocol for measuring sister chromatid exchange or suggestions for a commercial kit available to purchase.

07 August 2014 7,598 3 View

How does one prepare 300 uM hydrogen peroxide from 3 wt % H2O2 solution ?

Confused trying to change weight % into molarity using sigma's hydrogen peroxide.

06 July 2014 4,953 4 View

Has anyone used a protease to chop up a large protein like BRCA2 in order to detect by WB?

Having trouble detecting BRCA2 protein by Western Blot. I used a 3-8% Tris-Acetate gel, 40 ug of protein and ran the gel for 4 hours. To transfer I ran the wet apparatus at 4C overnight at 25...

05 June 2014 443 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View