Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

Do you know any journal calling for papers related to education during covid-19 and post covid 19?

I have recently completed a research based article related to Managerial Challenges and Opportunities posed by COVID-19 and would like to publish this in an international journal within a month...

01 January 2021 5,614 3 View

How can I convert my *.m file to Simulink?

When the main m file (script) is executed around 5 files are loaded and values are assigned to variables, four user defined functions are also called and output is plotted in the graph inside the...

23 November 2020 4,288 3 View

What is the most sensitive Non-invasive prenatal testing for fetal sex determination?

Is 8th week the earliest week where we can determine the fetal sex ?

05 October 2020 9,576 9 View

For in silico analysis of a variant, howmany bioinformatic tools are required to give a sign in the favour of its pathogenecity?

Dear fellows, I am wondering about, how many bioinformatic tools are required to declare a variant as pathogenic and deleterious?

02 October 2020 6,580 3 View

Which model either moment hinge rotation or fiber hinge model can dissipates more energy ?

While doing energy based seismic design with non- linear time history analysis in perform 3D, our main target is to find the maximum amount of energy dissipation from structural components?...

23 August 2020 4,764 2 View

Will there be any conflict of interest or other issues if you publish in a journal in which you are an editor?

Is publishing in a journal in which you are an editor (associate or chief editor) considered a good practice? Are such practices regarded as okay in academic circle?

03 August 2020 2,714 7 View

Can you suggest the compound that can inhibit CCR6 in the rat for in-vivo study?

CCR6 inhibitor for rat in-vivo experiment.

03 May 2020 5,655 1 View

Applying Plant extracts on cell line?

Has anybody tried applying plant extracts on MCF 7 cell lines after filtering them? will it (filtering) effect the antioxidant activity present in plants?

12 March 2020 5,258 3 View

No title

02 June 2019 8,134 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View