1% of Triton X-100 will be enough to cause lysis of cells . 

 Dear Asmita,

Thanks for your feedback. Just a quick one. I've read about 'macrophage lysis buffer' which requires much more recipe such as Tris buffer, NaCl, glycerol, EDTA, PMSF and protease inhibitor (Neil E. Reiner (2009). Macrophages and Dendritic Cells. Methods and Protocol. Springer Protocols). What's your opinion? 

I am agreed with Asmita, 1% of Triton is good enough. It all depends what you want to do with lysate afterwards. If you want measure enzyme activity, then you don't  add the protease inhibitors cortail in the buffer.

Hi Laurie,

Thanks for your reply. I will use the lysate for arginase assay.

Dear Shamsul, in my earlier work, i used 1% Triton X-100 for permeabilisation of membrane 

Dear Dr Nooraain,

Yes, it works for me now. Previously, I'm using a low concentration of Triton X-100, but complete lysis was not achieved on this 'superglue' cells.

Thanks

Dear Shamsul,

All the best and good luck. Assalam.