Do buffers need to match for DNA (aptamer) to protein binding in SPR on NTA chip?

Hello, As a newbie to SPR, I've been having problems with optimizing aptamer-protein binding on an NTA chip. My protein has a his-tag on it. Any help would be greatly appreciated. Question 1:...

14 June 2020 4,586 3 View

What is the C45 concrete damage plasticity parameters for abaqus ?

Since I am preparing my fyp, but really don't how to find the value of C45(eurocode) to run ABAQUS to check RC column.... Then, I am first to use this software, please help... Thanks a lot..

02 June 2020 6,604 7 View

What is the best model for this dataset?

Hi, I'm trying to do some IoT device classification, the dataset I'm using looks like this: 100 classes 5000~15000 samples for each class 10~15 features per sample Can you suggest the most...

16 April 2020 1,719 9 View

Which panel of immune cell subtypes you would analyze in Intestine and MLN of DSS-induced colitis mice?

I am looking for a panel of immune cell subtypes that in your opinion are fundamenatl to understand the possible immune imbalance in the intestine and mesenteric lymph node of DSS-induced colitis...

28 March 2016 1,330 3 View

Can anyone share a general protocol of Western blot using Urea/Thiourea/CHAPS lysis buffer?

14 February 2016 459 2 View

Any suggestion on the problem of nuclear membrane lysis and false-positive of IgG negative control in Chromatin immunoprecipitation (ChIP assay ) ?

18 March 2015 9,472 3 View

Is it really the case with General Hort books that still indicate the roots as the organs where the majority of reserves accumulate in trees ?

I have no direct measurement, but tree trunks and branches seem to me a lot more important in terms of reserves for their position and size relative to roots.

27 December 2014 9,581 10 View

Does anyone have examples of works, researches, or publications on digital mapping from UAV?

I know that UAVs are one of the most important innovation of the last years in geomatic and I know that with these systems it is possible to obtain 3D models and orthoimages with high levels of...

13 September 2014 6,186 9 View

Which is the best control label for cells obtained by enzyme digestion of vein endothelium for flow cytometry (FACS)?

I need know which is the best (with references if possible) control label to use for FACS as control group for cells obtained by enzyme digestion process of porcine vein tissue. Is any way to...

20 July 2014 529 8 View

How can a mouse splenocyte be cultured?

I have isolated some mouse splenocytes by meshing the spleens through cell strainers (70um). However due to bad timing I have to wait for at least 2 days for the reagents to come. I wonder if I...

02 March 2014 4,238 5 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

What do you think about the stability of redox active ketoesters? Can I isolate this compounds?

Although I want to synthesize a redox active ketoester like 1,3-dioxoisoindolin-2-yl 2-oxo-2-phenylacetate, I have no idea about the stability of this kind of compounds. I know that some redox...

19 January 2021 5,672 3 View

Work up of ester?

What is the detailed procedure for work up in ester synthesis from acid chloride and alcohol? What organic solvent to use for extraction?

18 January 2021 8,649 6 View

Miniemulsion polymerization in organic solvents?

Hello everyone, I want to make some nanoparticles by free-radical copolymerization of some kind of monomers in a Miniemulsion state! However, my monomers are sensitive to hydrolysis by water and I...

22 December 2020 9,648 1 View

Can alcohol feeding treat respiratory infections in birds?

In discussion with some farmers and colleagues, I came to know that poultry birds are fed alcohols to enhance their production/performance and treat the respiratory infecions. Is there any...

13 December 2020 4,700 17 View

How to calculate the % yield/conversion of Sucrose fatty acid ester by using GC, GCMS, HPLC and other?

Hi All, How to calculate the % yield/conversion of Sucrose fatty acid ester by using GC, GCMS, HPLC, and others? Please suggest.

19 November 2020 2,645 2 View

Has anyone tried separating cis and trans isomers of fatty acids derivatized as their trimethylsilyl esters using N,O-BSTFA reagent on FAME columns?

I have not achieved separation of cis and trans fatty acids derivatized as trimethylsilyl esters using N,O -BSTFA on the DB-5 Column, but I prefer this derivatization method as it is rather...

18 November 2020 4,993 3 View

What is needed for Olive Oil Lipid Extraction?

I am doing my thesis and would like to extract triglycerides from Olives or Olive Oil. I will be analysing the amount of triglycerides that are present in the olives or Olive Oil I will be...

01 November 2020 8,314 5 View

Please, is there suitable solvent to fractionate fatty esters into medium molecular weight fatty esters and high molecular weight fatty esters?

I want to separate a complex mixture of esters into medium and high molecular weight esters using solvent extraction.

11 October 2020 9,891 2 View

Did anybody know about effect of aldehyde on spidermite?

What organic acids influence on spider mite?

11 October 2020 9,944 3 View