Do buffers need to match for DNA (aptamer) to protein binding in SPR on NTA chip?

Hello, As a newbie to SPR, I've been having problems with optimizing aptamer-protein binding on an NTA chip. My protein has a his-tag on it. Any help would be greatly appreciated. Question 1:...

14 June 2020 4,586 3 View

What is the C45 concrete damage plasticity parameters for abaqus ?

Since I am preparing my fyp, but really don't how to find the value of C45(eurocode) to run ABAQUS to check RC column.... Then, I am first to use this software, please help... Thanks a lot..

02 June 2020 6,604 7 View

What is the best model for this dataset?

Hi, I'm trying to do some IoT device classification, the dataset I'm using looks like this: 100 classes 5000~15000 samples for each class 10~15 features per sample Can you suggest the most...

16 April 2020 1,719 9 View

Can anyone suggest me a valid Asian and Caucasian face dataset?

I am looking for a complete Asian and Caucasian face dataset with neutral expressions.

10 December 2018 8,832 1 View

Which panel of immune cell subtypes you would analyze in Intestine and MLN of DSS-induced colitis mice?

I am looking for a panel of immune cell subtypes that in your opinion are fundamenatl to understand the possible immune imbalance in the intestine and mesenteric lymph node of DSS-induced colitis...

28 March 2016 1,330 3 View

Can anyone share a general protocol of Western blot using Urea/Thiourea/CHAPS lysis buffer?

14 February 2016 459 2 View

Any suggestion on the problem of nuclear membrane lysis and false-positive of IgG negative control in Chromatin immunoprecipitation (ChIP assay ) ?

18 March 2015 9,472 3 View

Is it really the case with General Hort books that still indicate the roots as the organs where the majority of reserves accumulate in trees ?

I have no direct measurement, but tree trunks and branches seem to me a lot more important in terms of reserves for their position and size relative to roots.

27 December 2014 9,581 10 View

Does anyone have examples of works, researches, or publications on digital mapping from UAV?

I know that UAVs are one of the most important innovation of the last years in geomatic and I know that with these systems it is possible to obtain 3D models and orthoimages with high levels of...

13 September 2014 6,186 9 View

Which is the best control label for cells obtained by enzyme digestion of vein endothelium for flow cytometry (FACS)?

I need know which is the best (with references if possible) control label to use for FACS as control group for cells obtained by enzyme digestion process of porcine vein tissue. Is any way to...

20 July 2014 529 8 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View