I have some environmental samples that contain both fungi and bacteria and I want to isolate only bacteria
What is the best way to do that and if I want to add antifungal in the media, what is the best and what is its optimum concentration?
I will be thankful, if help with papers.
Best Regards
Hi,
I would suggest use 50-100 ppm of nystatin that will reduce fungal density.
Regards
You can use Nystatin @50 mg/Lt of the media after sterilization just before pouring, a usual practice to isolate bacteria from a mix culture source.
As already mentioned by others, Nystatin can be used with satisfactory results.
Nystatin would give you a good results. We are using this for actinomycetes isolation. Cycloeximide is banned you may not get this.
Depends on what you want to isolate and their populations. Fungi and gram positive bacteria are usually at lower population levels than gram negatives. I found using simple nutrient agar was efficient in isolating gram negatives and 10 min heating (don't remember exact temp-there is literature out there) of your serial dilutions and then plating them eliminated most of the gram negatives and non-spore bearers. Acidified PDA is effective in reducing fungi. Focus on the microbe that you want to isolate, if you don't you will have thousands of isolates and trying to keep them alive is a huge task in itself.
As mentioned by the others, Nystatin in concentration of 50 mg/l can be effective.
I was in the same situation. In addition to the suggestions above you can check this paper in which they test Natamycin as fungicide to reduce the fungal charge on samples.
http://aem.asm.org/content/58/3/1064.full.pdf+html
You can aso try to culture your samples con an incubator setup at 37˚C.
I suggest to used 50 micrograms of nystatin, in addition to 50 micrograms of cycloheximide per liter of medium
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