Surjit Singh

7 Questions 25 Answers 0 Followers

Questions related from Surjit Singh

Is it possible that point mutation does not change the gene and protein expression but it affects the protein function?

Hello, I am working with a kind of mutated protein and it seems that at the transcriptional level there is no change in the gene expression compared to wild type where as in western blot I can...

01 January 2019 5,855 2 View

Pull down assay?

Hi, I want to study protein protein interaction based on pull down assay. I want to know how to generate antibody against my target protein?

08 August 2016 4,620 0 View

Does DH5 alpha carry T7 and SP6 promoter region?

Hello everyone, I am trying to clone 300bp of DNA in DH5 alpha but after clonin i saw double band on the gel. one the desiredband which i need to get and another band just above my desired band....

11 November 2015 9,830 4 View

Is it necessary to clear PhD enterance examintions to get enrolled in PhD programs?

I would like to ask whether is it applicable to judge a student integrity and knowledge on the basis of these enterance test conduct by the educational system of India to get admission in PhD...

06 June 2014 3,173 6 View

What are the different types of analysis that can be done using a 16S rRNA gene sequence apart from phylogenetic tree analysis?

I have three 16S RNA gene sequences of three different bacteria. I have done the phylogenetic analysis and also constructed its dendrogram. But I want to know whether anything else can be done...

03 March 2014 4,112 1 View

What is the best way to remove bacteria from soil?

I want to remove bacteria present in the soil. There are options for killing them but killing will not eradicate bacteria from soil. So, I would like to know whether anybody can suggest to me how...

02 February 2014 6,106 8 View

How to detect major antibiotic groups by chemical reaction?

I want to do qualitative analysis of different antibiotic present in a mixture

03 March 2013 7,930 1 View