23 Questions 21 Answers 0 Followers
Questions related from Mohamed Sebak
I'm asking about the optimum oxygenation level or range for bioreactor fermentation of some soil actinobacteria for production of natural products. Thanks in advance
03 March 2018 5,724 8 View
I have problem that ethyl acetate and acetone have some antimicrobial activity against some of my standards strains, so I shouldn’t use them for antimicrobial activity not to affect my results...
09 September 2017 7,158 4 View
I’d like to know how to prepare the standards strains broth in the best way and how to do the test and interpreter the results I know that it should be done in triplicates but I’m not sure about...
09 September 2017 5,439 12 View
What's the highest temperature could actinomycetes isolates tolerate and what's maximum time for these high temperatures Please anyone help
07 July 2016 9,165 4 View
I want to get sharp bands of PCR products on the gel electrophoresis without any tailings, primer dimers or non-specific products. What is the best concentration of both primers and DNA to get...
12 December 2015 9,700 7 View
I have some environmental samples that contain both fungi and bacteria and I want to isolate only bacteria What is the best way to do that and if I want to add antifungal in the media, what is the...
12 December 2014 1,547 10 View
If I want to have bacterial samples which can produce biologically active secondary metabolites, what is the best way to do that and what are the sources from which I can collect them? Please help...
10 October 2014 9,007 16 View
If I want to obtain isolates of actinomycetes which produce novel biologically active secondary metabolites, what is the best media for cultivation of these actinomycetes to help them produce...
10 October 2014 6,299 9 View
I had isolated some isolates suspected to be actinomycetes. What is the best method for identification of them biochemically? Please help if you can Best regards
10 October 2014 9,559 3 View
If I want to have bacterial samples producing novel biologically active secondary metabolites, what is the best way for selection of the media for cultivation of these bacteria to produce new...
10 October 2014 761 7 View
If I want to extract a large plasmid from bacteria which are more than 30 kb, what is the best kit to extract it?
06 June 2014 4,200 6 View
I have some gram negative isolates that I had tested for Antimicrobial activity production and a lot of them were having antimicrobial activity but after subculture of these isolates, many of them...
06 June 2014 7,365 6 View
If I want to extract the genomic DNA from bacteria, is the use of heating or boiling of bacteria an efficient method or should I try another method? The aim of extraction is PCR and sequencing/
04 April 2014 997 15 View
If I want to have a good yield of PCR without any unspecific bands and have my desired bands be strong, what should be the typical starting concentration of both my genomic DNA and primers in the...
04 April 2014 9,849 6 View
We have a big problem in our lab. After detection of the PCR product by agarose gel electrophoresis which contained the carcinogenic Ethidium Bromide, we face the problem of getting rid of it. We...
04 April 2014 3,632 6 View
If I want to make glycerol broth for my isolates, what is the best method to do that and what are the best proportions of the glycerol and the broth?
04 April 2014 1,918 8 View
If I want to make glycerol broth for my isolates, what is the best method do that and what are the best proportions of the glycerol and the broth? Please provide a detailed protocol, thanks.
04 April 2014 3,557 1 View
If I want to isolate microorganisms producing antimicrobial natural products, what is the best source for these microorganisms I should isolate from; clinical, environmental or both? Please...
03 March 2014 4,241 6 View
When I was performing a PCR for some genes and running the PCR products on the agarose gel, I observed the presence of tailing and the bands were strong. Do you think I needed to change my PCR...
03 March 2014 1,965 11 View
I mean if I want to detect if some clinical samples of bacteria have an antimicrobial activity against other microorganisms.
03 March 2014 7,680 16 View
If I have a mixture of different bacteria, what is the best way to identify them by biochemical reactions? I mean, does any manual or reference summarize the identification of different bacteria?
03 March 2014 2,899 10 View
Following a PCR, there were DNA bands on the agarose gel, but they were different than my specific band, as they appeared far from my target band.
02 February 2014 6,306 19 View
After detection of biologically active peptides with antimicrobial activity, we need to fully identify them and know if they are new.
02 February 2014 5,252 3 View
