Muthamil Iniyan

8 Questions 19 Answers 0 Followers

Questions related from Muthamil Iniyan

How do I find molecular formulas from a mass spectrum?

Can anyone suggest me a way to find the molecule formula from a mass spectrum. If you have a link or software means, please share it. Thanks in advance.

12 December 2014 3,344 7 View

Antibacterial activity loss during storage at 4 degrees C?

I have screened hundreds of actinomycetes strains with antibacterial activity against bacterial pathogens. The strains were stored at 4 deg C. After 4-6 months of storage I have lost the activity...

09 September 2013 2,084 7 View

Detection of diaminopimelic acid (DAP) using TLC?

Identification of cell wall type is an essential step for the characterization of actinomycetes. I have prepared the cell wall hydrolysate using 6N HCl and carried out the silica gel TLC with the...

09 September 2013 686 1 View

Storage of Bioactive Secondary metabolites?

I would like to know how to store the Crude and Purified metabolites. I have stored the crude methanolic extract of plant and microbial metabolites at -20 degree C and -80 d c. It was extracted,...

07 July 2013 8,584 21 View

Strain number information in IJSEM journal?

The type strain is CMU-PLA07T (=JCM 16380T, = BCC 38279T). Here CMU-PLA07 is Chiang Mai University and strain no, What is the meaning of T? What is the meaning of =JCM 16380T, = BCC 38279T? Can...

06 June 2013 1,740 2 View

Can any one help me "Screening strategies to identify new antibiotics. Butler MS, Cooper MA.Curr Drug Targets. 2012 Mar;13(3):373-87. "

Currently i am screening different actinomycetes for the antibiotic production. Could you please help me for the full text?

04 April 2013 5,449 4 View

For the purification of secondary metabolites which strategy is best to get a single molecule?

1. Crude - Column chromatography - TLC - RP.HPLC 2. Crude - TLC - Column chromatography - RP.HPLC 3. Crude - TLC - RP.HPLC 4. Crude - Column chromatography - Preparative HPLC Can anyone suggest...

04 April 2013 2,921 11 View

Can anyone suggest to me how to escape from fungal contamination in the actinomycetes laboratory?

I have problems in actinomycete culturing, maintaining and during incubation periods. I am using petri plates for the screening of actinomycetes. During the incubation periods in the incubator I...

03 March 2013 5,726 5 View