How to quantify the number of targeting ligands per nanoparticle ?

I prepared PLGA nanoparticles. Then I loaded anticancer drugs in PLGA nanoparticles. Then I attached targeting ligands like folic acid (FA) and hyaluronic acid (HA) separately to target the cancer...

01 February 2020 212 11 View

How to avoid the loss of dead cells in cell cycle analysis using flow cytometry?

I am doing cell cycle analysis using Propidium iodide dye in flow cytometry. My samples are 1) cells 2)cells+ silica 3) cells+ drug 4) Cells+ silica-drug The cells treated for 24 hrs with the...

11 December 2017 1,360 4 View

How to find amount of graphene attached with mesoporous silica nanoparticles?

I synthesised the mesoporous silica nanoparticles. Then I attached graphene nanoparticles with mesoporous silica nanoparticles. TEM images clearly show the attachment of graphene over mesoporous...

10 November 2017 4,203 22 View

How to understand percentage of cancer cell growth inhibition and percentage of cancer cell killing in the same graph?

The graph show cyctoxicity of different combination of chemo drugs on a cancer cell line. The graph compare the percentage of cancer cell growth inhibition and percentage of cancer cell killing...

06 July 2016 6,248 3 View

Thermogravimetric analysis (TGA) can be used to find the amount of drug loaded in nanoparticles?

I am working on mesoporous silica nanoparticles. One can find the loading and entrapment efficiency using UV method. For example, I did the TGA for mesoporous silica from 30-900 degree celsius and...

06 July 2016 9,535 9 View

Whether amount of loading time have an effect of drug loading in nanoparticles?

I am working on nanoparticles for the treatment of cancer. I am working with hydrophobic and hydrophilic drug. usually people (in papers) use 24 hrs for loading of drug in nanoparticle. My...

01 January 1970 7,936 8 View

How to dissolve mesoporous silica internalized in cancer cells to quantify the amount of silicon content by using ICP-AES analysis?

I am working with mesoporous silica nanoparticle for the treatment of cancer. I am interested to know how much amount of mesoporous silica nanoparticles internalized in cancer cells. I seed 1...

01 January 1970 4,942 5 View

Whether PH play an important role in loading of drug into the nanoparticles?

If we change the PH of the solution from 7.4 to 9, it will affect the drug loading percentage in nanoparticles.

01 January 1970 4,464 4 View

How to coat polyethylene glycol over the surface of the mesoporous silica nanoparticles?

I am working on mesoporous silica nanoparticles for the treatment of pancreatic cancer. I am planning to put PEG over the surface of the mesoporous silica nanoparticles. I want good suggestions...

01 January 1970 10,018 3 View

Gemcitabine HCl is not loading into mesoporous silica nanoparitciles?

01 January 1970 6,567 8 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View