I am working on mesoporous silica nanoparticles for the treatment of pancreatic cancer. I am planning to put PEG over the surface of the mesoporous silica nanoparticles. I want good suggestions from researchers.
The easiest way is to use mPEG5K-Silane from Sigma or Jenkem. If you want to attach something to the final end of the PEG moiety, this procedure will not work. It is good only to pegylate the surface.
Thanks for your answer. For example, If I synthesise the mesoporous silica and thereafter I load the drug in mesoporous silica. Then I attach the PEG-silane to Mesoporous silica. Then I want to attach targeting ligands like transferrin or folic acid. I want to know how to attach the targeting ligand to PEGylated mesoporous silica. Looking for good suggestions.
You can use APTES to functionalize your surface with amino groups, then add glutaraldehyde. Add diamine-peg and finally add your protein and reduce with NaCNBH4.
For folic acid, I will use the same procedure up to diamine-peg (add NaCNBH4). Then add a carbodiimide and folic acid.
You will need to look up for reaction conditions as they will depend on the available NH2 groups on the surface and your biofunctionalization needs.
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