Prabhuraj R S

11 Questions 21 Answers 0 Followers

Questions related from Prabhuraj R S

How to quantify the number of targeting ligands per nanoparticle ?

I prepared PLGA nanoparticles. Then I loaded anticancer drugs in PLGA nanoparticles. Then I attached targeting ligands like folic acid (FA) and hyaluronic acid (HA) separately to target the cancer...

02 February 2020 305 11 View

What is the advantage of intraperitoneal injection over intravenous injection in mice?

I am working on nanoparticles for the treatment of cancer. We are planning to do antitumor efficacy in mice. People generally use intravenous route to inject nanoparticles to mice. I would like to...

04 April 2019 4,997 4 View

How to avoid the loss of dead cells in cell cycle analysis using flow cytometry?

I am doing cell cycle analysis using Propidium iodide dye in flow cytometry. My samples are 1) cells 2)cells+ silica 3) cells+ drug 4) Cells+ silica-drug The cells treated for 24 hrs with the...

12 December 2017 1,422 4 View

How to find amount of graphene attached with mesoporous silica nanoparticles?

I synthesised the mesoporous silica nanoparticles. Then I attached graphene nanoparticles with mesoporous silica nanoparticles. TEM images clearly show the attachment of graphene over mesoporous...

11 November 2017 4,271 22 View

Thermogravimetric analysis (TGA) can be used to find the amount of drug loaded in nanoparticles?

I am working on mesoporous silica nanoparticles. One can find the loading and entrapment efficiency using UV method. For example, I did the TGA for mesoporous silica from 30-900 degree celsius and...

07 July 2016 9,625 9 View

How to understand percentage of cancer cell growth inhibition and percentage of cancer cell killing in the same graph?

The graph show cyctoxicity of different combination of chemo drugs on a cancer cell line. The graph compare the percentage of cancer cell growth inhibition and percentage of cancer cell killing...

07 July 2016 6,327 3 View

Whether amount of loading time have an effect of drug loading in nanoparticles?

I am working on nanoparticles for the treatment of cancer. I am working with hydrophobic and hydrophilic drug. usually people (in papers) use 24 hrs for loading of drug in nanoparticle. My...

01 January 1970 7,999 8 View

Whether PH play an important role in loading of drug into the nanoparticles?

If we change the PH of the solution from 7.4 to 9, it will affect the drug loading percentage in nanoparticles.

01 January 1970 4,527 4 View

How to coat polyethylene glycol over the surface of the mesoporous silica nanoparticles?

I am working on mesoporous silica nanoparticles for the treatment of pancreatic cancer. I am planning to put PEG over the surface of the mesoporous silica nanoparticles. I want good suggestions...

01 January 1970 10,089 3 View

Gemcitabine HCl is not loading into mesoporous silica nanoparitciles?

I am loading Gemcitabine HCl into mesoporous silica nanopariticles. I took 25 mg of mesoporous silica nanoparticles and 10 mg of drug for loading purpose. I used PBS 7.4 as a medium to load the...

01 January 1970 6,629 8 View

How to dissolve mesoporous silica internalized in cancer cells to quantify the amount of silicon content by using ICP-AES analysis?

I am working with mesoporous silica nanoparticle for the treatment of cancer. I am interested to know how much amount of mesoporous silica nanoparticles internalized in cancer cells. I seed 1...

01 January 1970 5,004 5 View