Please explain the meaning of Single Pass Read. Why do some database contain only single pass reads?
We have obtained some novel miRNA sequences of buffalo, following NGS of sRNA sequences (Ion Torrent platform). I have contacted the miRBase however, no communications received. Please let me know...
05 June 2015 9,053 9 View
We have got an extensive list of IsoMir (cow genome used as map-file) after analyzing miRNA-seq data by miRAnalyzer (data were custom analyzed by s sequencing agency). Now we have the list in the...
05 June 2015 4,728 2 View
We have sequenced miRNA of bubaline/buffalo leukocytes. Some novel miRNAs have been obtained. One example file has been attached. We are now blasting each of the novel miRNA sequence against the...
02 March 2015 5,983 5 View
02 March 2015 459 2 View
I am a novice in this field. Please help me identify the mistake in this code to use GetOptions. I am using Windows OS (Win8.1) #!/C:/Perl64/bin/perl require 5.16.3; # use strict; use...
05 June 2014 302 6 View
Suppose I have got a pair of sequences (100 amino acid each), one is conserved cds and another one is a non-conserved coding sequence. 1. Which one should be used for phylogeny? 2. Do these two...
05 June 2014 4,340 12 View
Look at any phylogenetic tree we will find the internal nodes. However, do we have the common ancestors which are occupying those internal nodes in the real world? Sometimes we come up with...
03 April 2014 8,534 6 View
It is well known that bioinformatics uses the knowledge of molecular biology, statistics, computer science and information technology (a part of computer science only). Can we say bioinformatics...
03 April 2014 7,059 7 View
Why can lymphocytes be cultures in vitro, but not the other type of blood cells, like leukocytes (viz. granulocytes and monocytes) and erythrocytes? Although certain reports are there regarding...
07 August 2013 2,679 0 View
How useful is this site? http://bioinformaticssoftwareandtools.co.in/
07 August 2013 7,489 33 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed
30 July 2024 1,061 5 View