Please explain the meaning of Single Pass Read. Why do some database contain only single pass reads?
We have obtained some novel miRNA sequences of buffalo, following NGS of sRNA sequences (Ion Torrent platform). I have contacted the miRBase however, no communications received. Please let me know...
05 June 2015 8,950 9 View
We have got an extensive list of IsoMir (cow genome used as map-file) after analyzing miRNA-seq data by miRAnalyzer (data were custom analyzed by s sequencing agency). Now we have the list in the...
05 June 2015 4,620 2 View
We have sequenced miRNA of bubaline/buffalo leukocytes. Some novel miRNAs have been obtained. One example file has been attached. We are now blasting each of the novel miRNA sequence against the...
02 March 2015 5,891 5 View
02 March 2015 364 2 View
I am a novice in this field. Please help me identify the mistake in this code to use GetOptions. I am using Windows OS (Win8.1) #!/C:/Perl64/bin/perl require 5.16.3; # use strict; use...
05 June 2014 191 6 View
Suppose I have got a pair of sequences (100 amino acid each), one is conserved cds and another one is a non-conserved coding sequence. 1. Which one should be used for phylogeny? 2. Do these two...
05 June 2014 4,236 12 View
Look at any phylogenetic tree we will find the internal nodes. However, do we have the common ancestors which are occupying those internal nodes in the real world? Sometimes we come up with...
03 April 2014 8,435 6 View
It is well known that bioinformatics uses the knowledge of molecular biology, statistics, computer science and information technology (a part of computer science only). Can we say bioinformatics...
03 April 2014 6,954 7 View
Why can lymphocytes be cultures in vitro, but not the other type of blood cells, like leukocytes (viz. granulocytes and monocytes) and erythrocytes? Although certain reports are there regarding...
07 August 2013 2,594 0 View
How useful is this site? http://bioinformaticssoftwareandtools.co.in/
07 August 2013 7,391 33 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View