Is it correct to conduct two-way ANOVA when one combination of factors has only zero values?

Hello all! I have some problems while analyzing my data using two-way ANOVA approach. I have 2 factors (one with 2 levels and the other one with 4 levels) with 3 repetitions in each factor...

01 February 2019 9,131 6 View

More chl c than chl a content in brown algae?

Hello everybody! I was extracting pigments (chl a, c and fucoxanthine) from gametophytes cultures of Undaria pinnatifida using DMSO as a solvent. After quantification, my results showed more chl...

20 March 2018 3,186 3 View

More chl c than chl a content in brown algae?

Hello everybody! I was extracting pigments (chl a, c and fucoxanthine) from gametophytes cultures of Undaria pinnatifida using DMSO as a solvent. After quantification, my results showed more chl...

02 March 2018 5,256 4 View

What is the most suitable medium for testing axenic condition in macroalgae cultures?

06 July 2017 4,566 3 View

How can I correct baseline shift in an in vivo absorption spectrum?

Hi everybody!I was making a in vivo absorption spectrum from cultured filamentous brown algae and the spectrum that I have obtained is really similar with previous reports in this kind of algae....

11 July 2016 2,155 6 View

How can I correct baseline shift in an in vivo absorption spectrum?

Hi everybody!I was making a in vivo absorption spectrum from cultured filamentous brown algae and the spectrum that I have obtained is really similar with previous reports in this kind of algae....

06 July 2016 7,392 7 View

What is the difference between biological replicates, repetitions and replicates?

Hi everybody!I was checking some books and papers related to biostatistics and I found a no clear difference between biological replicates, replicates and repetitions (especially, between the two...

05 June 2016 2,344 13 View

What kind of mixture design can I use to know the best proportions of enzymes for protoplast isolation?

Hi everybody!In my research, i m isolation protoplast from Undaria pinnatifida gametophyte using cellulase (2%) and alginate lyase (3 U/ml). I was thinking to use a mixture design in order to know...

15 February 2016 4,408 2 View

A Laminariales gametophyte culture could be considered as a true cell suspension culture?

Hi everybody!I would like to ask your opinion (especially phycologist or anybody who works with plant cell suspension cultures) about this: as we know, Laminariales gametophytes (brown algae)...

01 February 2016 570 0 View

What kind of mixture design can I use to know the best proportions of enzymes for protoplast isolation?

Hi everybody!In my research, i m isolation protoplast from Undaria pinnatifida gametophyte using cellulase (2%) and alginate lyase (3 U/ml). I was thinking to use a mixture design in order to know...

01 February 2016 4,697 3 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View