What is insoluble in chlorform methanol solution?

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Livro DNA Sequencing Protocols de Colin A Graham ?

Gostaria de saber se alguém teria este livro em pdf, o DOI ao qual tive acesso não gerou resultados.

11 June 2024 4,042 0 View

What is the best fertilizer to use when growing potatoes?

Add

18 April 2024 8,327 3 View

Any suggestions for neuronal transfections?

I am currently culturing hippocampal neurons isolated from E18 rats and grown in Neurobasal medium (not the plus version) supplemented with B27 Plus. I transfect these neurons around DIV9 with...

06 November 2023 7,284 0 View

How does collaborative approach help clinical supervision of teachers?

contextualize in Caribbean setting, comparing research done in other parts of the world

18 October 2023 5,341 0 View

Hi, can someone please enlighten me on why we use IL-12 and IL-18 stimulation on human NK cells for checking IFN-gamma production?

I want to check the cytotoxicity by measuring IFN-gamma production of human primary NK cells after co-incubation with a fungal pathogen.

27 June 2023 9,092 2 View

Theory behind the Liebermann-Burchard assay for saponin determination?

Hi all I am interested to determine the saponin content from oily seeds and have found a few methodology from papers that uses Liebermann-Burchard assay (via UV-Vis spectrophotometer), however I...

15 May 2023 4,889 0 View

Extraction solvent of saponins from oily seeds?

Hi all I am currently embarking a project to determine saponin content in oily seeds, I have been looking at various literature methodology and according to available data in the literature,...

15 May 2023 1,261 1 View

Is sigma square considered model parameter in simple linear regression?

Some inclue only slope and intercept, but some include variance too as a model parameter. what is right?

10 March 2023 796 1 View

Hi, does anyone have experiences with the transfection of Primary Nk cells?

I want to do it but I'm a bit concerned about the viability at the end.

12 February 2023 7,114 2 View

SPSS statistical test advice?

Hello, I hope you are all well. I am finishing my MSc and am struggling quite a bit with organising/ analysing my data. I have conducted a study looking at the effect of different model sizes on...

15 August 2021 7,558 3 View

What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to explain the different interaction between soluble and insoluble polymer in water?

Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.

29 July 2024 6,350 3 View

How to remove/ replace trifluoroacetate countertop after deprotection?

After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...

29 July 2024 9,753 3 View

What is the solubility of Iron (+3) in Bismuth Oxide?

Dear all: I am modifying Bismuth Tungstate with Hematite (Fe2O3), so I would like to know about how much Iron(+3) can form a solid solution in a Bismuth Oxide matrix. Best wishes Marco

29 July 2024 2,163 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

How to dispose off lipids waste?

I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...

25 July 2024 637 5 View

Comparison of Methanol and ethanol treatment on collagen based scaffolds?

Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you

24 July 2024 3,937 0 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

Why methanol and sulphuric acid used in the analysis of polyhydroxyalkanoates (PHA) by GC-MS?

Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?

22 July 2024 1,210 2 View