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Questions related from Natasha Worden
I am interested in finding a piece of equipment that can perform electrofusion of plant protoplasts efficiently. Does anyone have an apparatus they have had success with and can recommend?
19 July 2021 6,681 4 View
I am trying to perform protoplast fusion and I am looking to buy an apparatus for electrofusion. Could I use an eporator used for electroporation?
26 April 2021 7,233 2 View
I am incubating cells in RPMI in a CO2 incubator at 37C and 80% RH. The phenol red started as a pink color and after 24 hours in the incubator it has faded. The color is more or less the same,...
03 July 2019 449 2 View
I am treated PBMCs with various compound to observe cytokine expression using ELISA, however, I am getting high expression in my untreated control cells as compared to my media-only control. I am...
06 June 2019 2,327 13 View
I am using cell culture supernatants in an ELISA. The cells are incubated at 37C, centrifuged, then the supernatants are used in an ELISA at RT. Why does the supernatant collection need to be at...
05 June 2019 8,697 3 View
I incubated plant material grindate in chloroform:methanol 2:1solution then filtered it to remove the lipid layer. What is left in the insoluble material? Carbohydrates and protein? and is...
07 May 2019 4,627 2 View
I am screening products against PBMCs, testing for potential immune activation. I have been looking at CD69 as a good general marker of immune activity, but would prefer something secreted. Does...
27 February 2019 4,170 6 View