Natasha Worden

7 Questions 8 Answers 0 Followers

Questions related from Natasha Worden

Can anyone recommend a good aparatus for performing protoplast fusion?

I am interested in finding a piece of equipment that can perform electrofusion of plant protoplasts efficiently. Does anyone have an apparatus they have had success with and can recommend?

19 July 2021 6,680 4 View

Can eporators used for electroporation also be used for electrofusion?

I am trying to perform protoplast fusion and I am looking to buy an apparatus for electrofusion. Could I use an eporator used for electroporation?

26 April 2021 7,232 2 View

Is it typical for phenol red to fade over time during cell culture?

I am incubating cells in RPMI in a CO2 incubator at 37C and 80% RH. The phenol red started as a pink color and after 24 hours in the incubator it has faded. The color is more or less the same,...

03 July 2019 447 2 View

What could cause high cytokine expression in untreated PBMC cell culture?

I am treated PBMCs with various compound to observe cytokine expression using ELISA, however, I am getting high expression in my untreated control cells as compared to my media-only control. I am...

06 June 2019 2,324 13 View

Why does the collection of cell culture supernatants need to be performed at 4C?

I am using cell culture supernatants in an ELISA. The cells are incubated at 37C, centrifuged, then the supernatants are used in an ELISA at RT. Why does the supernatant collection need to be at...

05 June 2019 8,690 3 View

What is insoluble in chlorform methanol solution?

I incubated plant material grindate in chloroform:methanol 2:1solution then filtered it to remove the lipid layer. What is left in the insoluble material? Carbohydrates and protein? and is...

07 May 2019 4,624 2 View

Can anyone recommend a good marker for lymphocyte activation?

I am screening products against PBMCs, testing for potential immune activation. I have been looking at CD69 as a good general marker of immune activity, but would prefer something secreted. Does...

27 February 2019 4,167 6 View