Hi, can someone please enlighten me on why we use IL-12 and IL-18 stimulation on human NK cells for checking IFN-gamma production?

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Malcolm Nobre

Hello Fariha Natasha

IL-18 is required to prime NK cells to produce IFN-γ in response to IL-12. Combined stimulation with IL-18 and IL-12 (IL-18 + IL-12) has a powerful synergistic effect on NK cell activation and triggers an important immune response to infections.

I have attached a few articles for your reference.

Article Combined Stimulation with Interleukin-18 and Interleukin-12 ...

Article Cutting edge: Priming of NK cells by IL-18

https://pubmed.ncbi.nlm.nih.gov/11801649/

Article Favorable in vitro effects of combined IL-12 and IL-18 treat...

Best.

Fariha Natasha

Hi Malcolm Nobre, Thanks a lot for your answer and the recommended articles.

Regards