How to estimate charge transfer and polarization resistance in a battery?

Hi. I have performed many EIS experiments using a cylindrical battery at different temperatures and state of charge. Some results (near SOC=0) are as the attached figure. How can I find the...

08 July 2019 1,746 2 View

Are there different types of Dansyl Chloride?

I was using Sigma Aldrich Dansyl Chloride (03641) suitable for HPLC derivatization on a method for quantification of hormones, but then I changed it for product (39220) for fluorescence and now I...

24 June 2019 1,484 3 View

E.coli cryopreservation for routine subloning at -20?

I would like to know if there is any protocol or personal experience to cryopreserve chemically competent E.coli cells (for routine subloning) at -20C. The only ultra-freezer that I have access is...

20 December 2018 3,718 3 View

E.coli cryopreservation for routine subloning at -20?

I would like to know if there is any protocol or personal experience to cryopreserve chemically competent E.coli cells (for routine subloning) at -20C. The only ultra-freezer that I have access is...

11 December 2018 1,592 3 View

Loss of internal standard in a LC ESI MS/MS protocol for Gliclazide?

We are performing an LC ESI MS/MS protocol to quantify Gliclazide on plasma samples. We have noticed that the internal standard (Gliclazide D4) has a very high variation compared with gliclazide....

22 July 2018 8,936 4 View

Do anyone Know if there is some procedure to perform Lumsdaine and Papel test for unit roots breaks in stata or gretl?

In RATS you perform LPUNIT procedure. I want to know if I can do in other statistical software

27 September 2017 4,208 2 View

How can I align images in a z-stack in image j in order to make reconstructios of biocytin labeled neurons?

I took pictures of some neurons I want to reconstruct using ImageJ; however, they are not in the same x and y plane. I can built a z-stack without problems, but of course, they do not appear...

20 June 2017 7,704 4 View

Which are the difference between luciferase promoter assay and overexpression assay?

I have many evidences that a LncRNA expression is correlated with a near gene. Coding sequence of LncRNA and gene are separated by 1700 pb. This sequence included the promoter of gene (nothing is...

20 July 2016 9,848 1 View

Hi, could someone please help me with the identification of a Palaemonid shrimp, genus: Leander?

10 July 2016 985 2 View

How can I eliminate interferences of some ions from carbonate and bicarbonate analysis?

Hello everyone I will do a validation of carbonates and bicarbonates analysis in soils (through titration analysis) but I have found that would be some interferences of some ions present in the...

17 April 2016 5,852 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View