I´m looking for any paper or help with half-life of these mRNA from cell culture of PBMC
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
22 July 2024 8,770 1 View
Hello, I've been doing free floating IHC using a VAChT antibody with a 1:1k dilution (139 103 - Synaptic Systems). However, there has been a struggle to keep an even stain with my samples of...
06 July 2024 4,521 2 View
Me interesa investigar la distribución espacial de los gremios tróficos de aves en un área natural específica de bosque seco tropical, analizando la abundancia relativa de cada gremio en las...
12 June 2024 9,313 1 View
I've been looking for software that simulates the integration of passenger and freight transportation systems. For instance, tools like Vissim and Visum primarily focus on modeling passenger...
14 May 2024 9,283 2 View
I am interested in the study of dendritic cells response under infection with mutant strain candidate to vaccine against TB. But I am begginer in the work with dendritic cells, somebody can help...
20 March 2024 1,587 7 View
Hello to everyone. I ran into this issue that most likely is a simple one. But, when performing the Sørensen-Dice index, what happens when there's no shared species with lower abundance? I mean, I...
27 February 2024 6,555 2 View
How can I seed 0.5x10^6 cells per well in a 96-well plate (I only need to seed 5 wells)? I have a T25 flask (5 mL total volume) with a cell density of 0.6x10^6 cells/mL.
17 February 2024 5,535 1 View
I am having a problem with the reproducibility of the endogenous controls of my samples in qPCR. I have tested several concentrations of cDNA with the objective of discharging that this was the...
31 January 2024 9,833 5 View
Hello everyone, I have a question about properties of 2 different solvents. For little context, I have a homework about liquid-liquid extraction so I had to compare which solvent is better for the...
16 November 2023 4,662 1 View
Hi everyone. I'm in the process of purchasing an stereo-microscope of the Amscope brand, specifically an SM-2T, but I ran into this thought of what if this microscope is not suitable for what...
04 October 2023 4,274 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View