Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

What could be the possible causes of DNA degradation after extraction from 4% gel?

Hi everyone, During small RNA library preparation, I run the samples in 4% agerose gel to select the band corresponding to my target. I established beforehand the protocol and it works fine....

02 February 2021 5,008 2 View

What is this citation style called?

Hello everyone, I am preparing a manuscript for a journal which indicates in the authors guidelines that the citation and referencing style should look like the following: Citation in the text...

22 September 2020 6,897 6 View

What would be the effect of Spike-In control in microRNA Next Generation Sequencing results?

Hello everybody, I am currently extracting total RNA including microRNA from serum using miRNeasy Serum/ Plasma kit Catalog Number 217184 from Qiagen. Within the procedure, we add a Spike -In...

07 August 2019 5,494 3 View

What is the concentration of Gemcitabin that I should used to treat PT45P1 pancreatic cancer cell lines?

Hello everyone, Is there anyone has experience in treating PT45P1 pancreatic cancer cell lines with Gemcitabin? I would really appreciate it very much if you let me know how much the Concentration...

07 August 2019 5,731 3 View

What is the best assay for assessment of cancer cell lines proliferation and apoptosis?

I working with pancreatic cancer cell lines PT45P1 and would like to know whether there is a better test rather than wound healing assay to assess the cellular proliferation after and before...

07 August 2019 8,401 10 View

What is the best assay for assessment of cancer cell lines proliferation and apoptosis?

I working with pancreatic cancer cell lines PT45P1 and would like to know whether there is a better test rather than wound healing assay to assess the cellular proliferation after and before...

06 August 2019 6,195 9 View

How we can prepare labeling, washing and incubation buffers for antibody micro-array?

I need to prepare a washing, incubation and labeling buffers for antibody microarray. Anyone has idea how to prepare them?

09 October 2018 6,505 1 View

What the best protocol for protein extraction from human serum?

I am planning to extract the whole serum protein from serum samples for further microarray analysis. what is your recommendations please. a good protocol is highly appreciated. Thanks in advance

16 September 2018 3,896 3 View

What the best protocol for protein extraction from human serum?

I am planning to extract the whole serum protein from serum samples for further microarray analysis. what is your recommendations please. a good protocol is highly appreciated. Thanks in advance

08 September 2018 9,076 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

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02 March 2021 3,060 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View