Can anyone suggest why CD47 expression was higher in old RBCs concentrates with leukocytes than in RBCs concentrates without leukocytes?
(leukodepleted)?
Is there macrophage in old RBCs concentrates with leukocytes? If macrophages are present in the concentrate, perhaps erythrocytes are induced by macrophages to express CD47
This result looks strange.
It is well documented that leucodepletion induced improving of stored RBCs properties. Kamel et al demonstrated that increasing of CD47 expression correlated (r = -0.574) with decreasing of WBC concentration in the unit, see http://www.bloodtransfusion.it/Articoli/59/En/131-09_EN.pdf.
Does you compare units that stored equal period in similar stored solution?
First , recheck the calibration of your machines. Then , try to optimize the CD47 expression with different concentration of EBC : WBC ratio and then get it confirmed.
As someone told before, macrophage tend to phagocytose the RBC , so that RBC with Macrophage need to express more " don't eat signal " cd47 to prevent pahocytosis by macrophage.
Though , nowadays, a new complex of CD47 - SIRPa are seen to initiate phagocytosis.
http://journal.frontiersin.org/article/10.3389/fphys.2014.00009/full
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