Is there a dataset available on actual individual person usage of OS? If not what is the best way to create it?

I want to create a N.N which can learn person OS usage based on their use of application and data related to specific application's background time, usage frequency, usage time of the application...

07 December 2020 1,252 4 View

How can i simulate loss in each layer of perovskite in Silvaco TCAD?

I am simulating a Perovskite solar cell to optimize it for best possible efficiency. I want to see losses in each layer of the perovskite solar cell, absorber, HTL and ETL and TCO . Does anybody...

15 June 2020 252 5 View

In which solvent CdS-TiO2 composite may dissolve?

want to perform CV measurement.

14 August 2018 5,773 2 View

How Gray level co-occurrence matrix or GLCM calculated for an image is implemented on that image for texture feature extraction?

Information such as contrast, entropy, dissimilarity index and variance are computed to know the properties of any image, but how these values are used to for further classification or feature...

12 October 2017 4,978 3 View

Entering the field of Data Science as a developer

Being a python programmer, I have decided to get in the field of data science and later on AI and Machine Learning. I have already brushed up the knowledge of statistics and other python libraries...

23 August 2017 5,999 13 View

What are the assay I should use for insulin receptor activation on NIT cell lines?

We have NIT cell lines, what are the assay should I use for checking insulin receptor activation ?

10 May 2017 9,836 1 View

What assay I should perform for insulin receptor activation?

I want to check anti diabetic effect by insulin receptor activation. Which assay i could perform to check insulin receptor activation.

26 April 2017 1,835 2 View

Need help in culturing pure Keratinocytes..!!

25 September 2016 4,578 7 View

How can I detect level of phosphorylated PRDX3 in protein samples?

I wanna check the PRDX3 (Peroxiredoxin) and Phosphorylated PRDX3 level in my protein samples, so for that purpose I require antibodies for performing Western blot. The antibody for PRDX3 is...

29 November 2015 8,468 6 View

Reciprocating pumps v/s Centrifugal Pumps

Reciprocating pumps have many disadvantages like high cost of installation, bulky size, high maintenance cost, non uniform torque, etc. but they are still being used in industries. Could anybody...

13 July 2012 1,013 23 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View