70% ethanol or Isopropanol is commonly used disinfectant in cell culture lab. Apart from that what is the best fungal disinfectant for mammalian cell culture. is H2O2 effective against fungus??
Hello, Krisna!
for hydrogen peroxide:
Article The Effect of Hydrogen Peroxide on the Growth of Microscopic...
Use of Antibiotics and Antimycotics
https://www.thermofisher.com/ru/ru/home/references/gibco-cell-culture-basics/cell-culture-protocols/use-of-antibiotics-and-antimycotics.html
I had spores that I could not kill completely with ethanol. Autoclaving is reliable, but only for glassware and those few plastics that can take the heat.
If you have a plastic that does not survive autoclaving, it's best to throw it out. Cell culture plastics are sterilized by gamma irradiation at the factory, and should be treated as disposable by the end user.
With reactive chemicals like H2O2 or bleach, the problems is that they can attack the plastic, or alter its surface in subtle ways that you can't easily get rid of or test for.
Hello Krisna. You can see the link..
https://www.researchgate.net/post/How_to_overcome_from_a_fungus_contamination_in_a_cell_culture
Fungus and mold spores are ubiquitous in the environment and generally infect cultures via an airborne route. Heating and air-conditioning systems are notorious for having high concentrations of spores. Therefore, the seasonal changes of fall and spring usually result in an increase in this type of contamination in cultures as heating or A/C systems are switched on or off. Also, particularly in the spring, the higher bioburden in the air from pollen particles can carry fungal spores into air handling systems and into labs on the clothes of lab personnel.The two most common antimycotic agents used in cell culture that are effective against fungi or molds are Amphotericin B (Fungizone) and mycostatin (Nystatin). Important note: routinely used antibiotics such as penicillin/streptomycin (pen/strep), gentamicin, and kanamycin are NOT effective against fungi or molds. Fungizone can be used in media at final working concentrations between 0.25ug/ml and 2.5ug/ml. Fungizone is typically very toxic in cell culture systems and should be used conservatively. Nystatin can be used at final working concentrations between 100U/ml and 250U/ml. Nystatin is a colloidal suspension rather than a solution and should be mixed thoroughly before it is added to cell culture media. When nystatin is in medium and viewed under a microscope, it will appear as small crystal-like particles. A very useful list of antibiotics, the organisms they are effective against, and recommended working concentrations compiled by the Sigma-Aldrich Company can be found as a pdf document by clicking on the following link.
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