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Questions related from Krisna Moorthi P
I have isolated RNA from PBMC of 5 different samples. After quantification , Each have different concentration. I will use it for qPCR experiments after converting it into cDNA. How much...
22 May 2021 4,066 4 View
I am using streptomycin to kill the extracellular bacilli while infecting THP1 derived macrophages with M.tb. Can streptomycin cross the macrophage membrane ?. I read some articles. It says that...
16 February 2021 1,986 3 View
I have glycerol stocks of M.tb in -80°C freezer. But it is not reviving properly; shall I rapidly thaw it? or Shall I keep in -30°C for one hour , 4°C for one hour and in room temperature (25...
20 January 2021 7,308 4 View
70% ethanol or Isopropanol is commonly used disinfectant in cell culture lab. Apart from that what is the best fungal disinfectant for mammalian cell culture. is H2O2 effective against fungus??
23 November 2020 10,123 5 View
While amplifying particular part of a gene, we are adding genomic DNA. But I observed only PCR products not genomic DNA. Why genomic DNA is not appeared in PCR product?
19 November 2020 9,465 8 View
Many literature showed that how to determine IC50 drug concentration using MTT assay. But I want to know how to determine non-cytotoxic concentration of a particular drug with maximum its...
01 November 2020 2,121 3 View
Brain Heart Infusion broth is suitable for fastidious organisms such as streptococcus, meningococcus, pneumococcus. But it is suitable for fungus?
05 February 2020 5,019 3 View
