How can we amplify DNA of a target gene of Plant pathogen by using qPCR?
Dear Researchers, I have read an article and there are two set of primer pairs. Sense primers and antisense primers. The paragraph from the article is as [ The partial DNA-dependent RNA...
31 December 2019 7,702 7 View
In 3ml, how can I make all these buffers, I have read this paper for SOD activity, Please find below [The 3 ml reaction mixture contained 50 mM potassium phosphate buffer (pH 7.0), 0.5 mM...
06 July 2019 3,836 6 View
Dear All, I need to check the growth promoting effects of some bacterial antagonists. I want to obtain the jasmonic acid and other growth promoting hormones. Then I want to do LCMS. Any step by...
05 June 2019 2,710 4 View
I want to check some metabolites from the root exudates or leaf extracts. Than I want to check the metabolites concentration like Jasmonic acid, via GC-MS, LC-MS or HPLC. Can anyone explain me...
03 April 2019 3,524 5 View
Dear All, Can anyone explain the pairwise permanova for the group 1 and Group 2. The data is given as below: Group 1 Group 2 Sample size Permutations pseudo-F p-value q-value A C 12 999...
11 December 2018 5,516 0 View
I have a haemocytometer with 25 major grids and each grid is divided into 16 small grids, The dept is 0.1mm. I put 10 ml ddH20 in petriplates and rub to get spores. I take 10ul from that 10 ml...
01 February 2018 2,835 1 View
Dear All, I have read several papers like these: twelve strains of fungus Trichoderma harzianum were evaluated against Phytopathogen. So in these case what would be the definition of strains?
10 November 2017 6,435 10 View
Dear All, I want to extract antibiotics and peptides from a fungi. Is there any proper procedure to extract antibiotics from the fungi.
10 November 2017 6,815 3 View
Dear All, I have cultured two fungi one 9cm petri plates. The growth of biocontrol agent is very slow but it has potential to parasitize a plant pathogen. I want to measure the percent inhibition...
10 November 2017 5,113 3 View
I run the Gell for DNA of some of samples. However some DNA bands were thinner some were more thicker in size and some of them were like smear from top to bottom. What could be the reason?
10 November 2017 9,962 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?
31 July 2024 5,087 4 View
What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?
31 July 2024 7,921 5 View