Could change of FBS (Fetal Bovine Serum) affect the quality of the cell?

10 November 2014 7,977 6 View

Do these colon cancer cells (HCT-116, COLO-205 and SW-620) express Her-1 and Her-2 receptors?

I am working in natural compound that has anticancer activity against Colorectal Carcinoma, I want to study the effect of this compound on receptor expression and activation, so I want to know if...

09 October 2014 2,432 1 View

How I should use the TGF-beta receptor 1 antibody to treat fibroblasts?

I am intending to use TGF-beta receptor 1 antibody as a blocker in cultured fibroblasts treated with TGF-b. In general, to assess the FMT (fibrobllast-to-myofibroblast transition), cells are...

05 June 2014 9,310 2 View

Do all proteins need a denaturation step to be used for an SDS-PAGE?

Sample denaturation is one of the steps that should be carried to prepare the proteins for SDS-PAGE. Should we carry this step out for all proteins? Or can some proteins be affected by this...

02 March 2014 7,414 14 View

Which Positive Control should I select?

I am targeting NF-kB pathway in cancer cells, and my drug may block this pathway. According to my hypothesis, I will use western blot and qRT-PCR to confirm this effect. Should I use a positive...

01 February 2014 6,968 10 View

How to induce Nf-kB pathway in cancer cell line?

I am working on a drug that is supposed to block NF-kB, so I want to study the effect of the drug on protein and mRNA expression in colorectal cancer cells. Should I induce NF-kB in these cell...

31 December 2013 5,193 7 View

Why should we use Trypsin without EDTA?

When we want to carry flow cytometry for cell cycle analysis or for apoptosis study as examples, we have to use Trypsin to harvest the cell without EDTA. Why do we have to use Trypsin without...

05 June 2013 5,712 2 View

Does the number of cell passages affect the activity of drug?

I am doing a cytotoxicity study for a group of drugs in different cell lines using an MTT assay. I am wondering about cell passage (Subculturing). One time I worked in an early passage (such as...

04 May 2013 1,795 6 View

Can I store the drug solution made in cell culture media?

I am using culture media to dissolve my drug as the drug is not stable in DMSO, so I am keeping fresh drug solution for when I want to carry any experiment (cytotoxicity, cell cycle analysis,...

04 May 2013 5,045 9 View

The cell is dying with unknown reasons.

I have worked in NCI-H1650 (human non-small cell lung cancer cells), I have thawed these cells for 3 times, in all the cells were ok and attached well after 24hr, but after 48hr the cell is dying...

02 March 2013 8,171 5 View

No title

02 March 2021 3,060 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View