Could change of FBS (Fetal Bovine Serum) affect the quality of the cell?

10 November 2014 7,977 6 View

How I should use the TGF-beta receptor 1 antibody to treat fibroblasts?

I am intending to use TGF-beta receptor 1 antibody as a blocker in cultured fibroblasts treated with TGF-b. In general, to assess the FMT (fibrobllast-to-myofibroblast transition), cells are...

05 June 2014 9,310 2 View

Do all proteins need a denaturation step to be used for an SDS-PAGE?

Sample denaturation is one of the steps that should be carried to prepare the proteins for SDS-PAGE. Should we carry this step out for all proteins? Or can some proteins be affected by this...

02 March 2014 7,414 14 View

Which Positive Control should I select?

I am targeting NF-kB pathway in cancer cells, and my drug may block this pathway. According to my hypothesis, I will use western blot and qRT-PCR to confirm this effect. Should I use a positive...

01 February 2014 6,968 10 View

How to induce Nf-kB pathway in cancer cell line?

I am working on a drug that is supposed to block NF-kB, so I want to study the effect of the drug on protein and mRNA expression in colorectal cancer cells. Should I induce NF-kB in these cell...

31 December 2013 5,193 7 View

Why should we use Trypsin without EDTA?

When we want to carry flow cytometry for cell cycle analysis or for apoptosis study as examples, we have to use Trypsin to harvest the cell without EDTA. Why do we have to use Trypsin without...

05 June 2013 5,712 2 View

What is a suitable cell line for studying antiangiogenic activity for a drug?

I am planning to check antiangiogenic activity for my compound, but I don't know what cells I should use to study the in-vitro and in-vivo antiangiogenic activity of this compound?

05 June 2013 3,683 4 View

Does the number of cell passages affect the activity of drug?

I am doing a cytotoxicity study for a group of drugs in different cell lines using an MTT assay. I am wondering about cell passage (Subculturing). One time I worked in an early passage (such as...

04 May 2013 1,795 6 View

Can I store the drug solution made in cell culture media?

I am using culture media to dissolve my drug as the drug is not stable in DMSO, so I am keeping fresh drug solution for when I want to carry any experiment (cytotoxicity, cell cycle analysis,...

04 May 2013 5,045 9 View

The cell is dying with unknown reasons.

I have worked in NCI-H1650 (human non-small cell lung cancer cells), I have thawed these cells for 3 times, in all the cells were ok and attached well after 24hr, but after 48hr the cell is dying...

02 March 2013 8,171 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

No title

02 March 2021 3,060 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View