In the laboratory, micro-organisms are usually grown or

cultured

in liquid medium (broth) or on solid medium (agar plates or slopes). Growth of bacteria and yeasts shows as cloudiness or turbidity in the broth although sometimes bacteria grow as a layer on the surface of the broth or at the bottom of the culture tube.he growth on plates depends upon how the plate has been inoculated.types of inoculations on agar plates

Subculturing

is the aseptic transfer of micro-organisms from a culture to fresh medium. he freshly inoculated medium is then incubated at the temperature appropriate for growing the organism.here are four subculturing procedures with which you should become familiar. hey are!

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"olid to solid! the transfer of bacteria or fungi from an agar slope or plate culture to an agar plate

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"olid to liquid! the transfer of bacteria or fungi from an agar slope or plate culture to a broth

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Liquid to solid! the transfer of bacteria or fungi from a broth culture to an agar slope or plate

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Liquid to liquid! the transfer of bacteria or fungi from a broth culture to a broth$ontainers of culture media to be inoculated must be labelled with initials, date and name of organism. o present possible confusion, plates are mar&ed on the underside while tubes and bottles must be labelled on the side.#ids are not labelle.

Following

It depend on the species of bacteria would you like to sub-culturing, use enriched media such as blood agar or Brain heart infusion agar, incubated under aerobic conditions at 37 °C, then inoculate these bacteria on it's selective media.

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Following

https://www.ncbi.nlm.nih.gov/pmc

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I agree with Ebtehal N. Mahmmoud

It depend on the species you want to isolate such as Staphylococcus aureus cultured on mannitol salt agar and staphylococcal no. 110@