Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

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23 February 2021 4,014 3 View

Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

How to find a particular aptamer complementary sequence?

I can find several papers that give me a guide for choosing a suitable aptamer sequence 1. But how to find a property aptamer sequence 2(partially complementary to sequence 1)?

09 February 2021 3,511 1 View

Do anyone have a full MATLAB code for structural topology optimization using Genetic Algorithm? Such as a cantilever beam?

Do anyone have a full MATLAB code for structural topology optimization using Genetic Algorithm? Such as a cantilever beam? Could you provide some MATLAB code to me , a beginner for GA. Thank...

03 February 2021 3,789 5 View

How to do DNA normalization based on qPCR values?

Hi Everyone: I have a question regarding to qPCR. I am trying to do RT-PCR, and I want to have equal amount of input DNA for my RT-PCR, so I normalize my DNA input based on qPCR values of a...

31 January 2021 6,977 4 View

MS/MS protein analysis?

Hi Everyone: I want to quantify the relative amount of a protein in 2 samples. I will first digest the sample with trypsin, and subject the resulting peptides to LC-MS/MS analysis. I will then...

25 January 2021 8,333 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Can we proceed to perform Two-way Mixed-ANOVA despite normality violations?

While testing assumptions, Shapiro-Wilk Test failed. However, I proceeded with the analysis and the interaction effect was significant. During our course- lectures, it was mentioned that Anova...

24 February 2021 228 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

Which test should I use in SPSS if I have two independent variables and one dependent variable that is measured using two tests?

I am researching the effect of gender and video game use on reaction times. To do this, I will have participants complete two reaction time tests (a simple test and a four choice test). I am a...

17 February 2021 9,343 4 View

Can I perform two way ANOVA in case of some missing groups?

I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results...

17 February 2021 8,933 4 View

Spss won't divide my independent variables into two levels when i run a two way anova test. can anyone explain why this is the case?

i have two IVs and four DVs, initially, i was going to run a two way manova test but my DVs are not correlated, so i was told it's better to do individual two way anova for each DV. however,...

16 February 2021 3,390 7 View

Arena Simulation help for choosing between two stations?

Was wondering if anyone here can help me out with a problem I've run into with the Arena Simulation software. Basically, the production process I am modelling has two stations. I want entities to...

13 February 2021 3,488 3 View

How to determine the significance of "diagonal" contrasts in two-way ANOVA interactions on SPSS?

Hi all, I'm running a 2 x 2 between-subjects ANOVA with the setup in the attached image (kindly refer to JPG attachment). I understand that calculating simple effects involves determining...

09 February 2021 547 3 View

Two-way ANOVA?

Does it make sense to use two-way ANOVA to compare response of two different organisms (definitely have different values for the dependent variable) exposed to stress levels? I thought to use...

08 February 2021 5,418 4 View

Difference between Second-harmonic generation and Two-photon absorption?

Hello Dear Researchers, I am confused about the Difference between Second-harmonic generation and Two-photon absorption? Can anyone describe the two phenomena briefly in the physical picture? What...

24 January 2021 9,912 5 View