Transfection toxicity when plated on glass coverslips?

I've been using A549s for the past couple of years to transfect in my protein of interest for imaging. Recently I've started to experience issues with transfecting cells seeded on glass...

27 January 2021 6,801 3 View

How can I obtain the expansion orbital coeffiecients in Hartree Fock program?

23 December 2020 6,756 3 View

Have you cloned a PCR product containing 2X2A sequences into a vector that contains another 2A sequence?

Hi! I am having problems cloning a 1.6 kb fragment containing 2 different genes and 2X2A peptides into a vector that I already used to clone other fragments (digested with PmeI/BamHI). The vector...

17 December 2020 5,218 3 View

How to validate CNVs (deletions and duplications) in human samples by qPCR?

Hello, I am interested in the validation of CNVs (deletions and duplications) in human samples by qPCR. We are trying to validate the primers for the experiments, but the primers are not working...

21 October 2020 5,896 2 View

Creating individual landuse transition maps?

Dear Fellows. I have created land-use maps of two time periods. I have created the change map which shows all the changes from one class to the other, however, I want to create the transition...

28 August 2020 9,172 2 View

What is the difference between KPIs and Balanced Scorecard ?

Measuring business performance Organisations face the intense pressure of carrying out their processes efficiently, effectively and sustainably every day (Ghosh & Fedorowicz, 2008). Companies...

30 March 2020 1,553 4 View

Is it ethical to analyze information on social media for research purposes?

I intend to study some health posts and comments on the social media with the intention of analysis such information for research purposes. I plan to maintain anonymity of the originators of the...

21 August 2019 9,213 3 View

Can I create a CitNet Explorer (VOS viewer) map using more than one "cvs" file or do I have to compile then?

Can I create a CitNet Explorer (VOS viewer) map using more than one "cvs" file or do I have to compile then?

02 March 2019 614 1 View

What kind of body measures can I take in wasps to contrast differences between individuals from different nests?

27 January 2019 7,520 11 View

What kind of body measures can I take in wasps to contrast differences between individuals from different nests?

31 December 2018 7,497 12 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View