Transfection toxicity when plated on glass coverslips?

I've been using A549s for the past couple of years to transfect in my protein of interest for imaging. Recently I've started to experience issues with transfecting cells seeded on glass...

27 January 2021 6,801 3 View

Creating individual landuse transition maps?

Dear Fellows. I have created land-use maps of two time periods. I have created the change map which shows all the changes from one class to the other, however, I want to create the transition...

28 August 2020 9,172 2 View

What is the difference between KPIs and Balanced Scorecard ?

Measuring business performance Organisations face the intense pressure of carrying out their processes efficiently, effectively and sustainably every day (Ghosh & Fedorowicz, 2008). Companies...

30 March 2020 1,553 4 View

Is it ethical to analyze information on social media for research purposes?

I intend to study some health posts and comments on the social media with the intention of analysis such information for research purposes. I plan to maintain anonymity of the originators of the...

21 August 2019 9,213 3 View

Can you still run a mixed ANOVA if the homogeneity of variances were violated for one of you within-group factors?

I am currently analysing data on whether there is a difference in the level of understanding between maths and science using a sample of year 1, year 2 and year 3 students. I am also looking at...

07 March 2018 2,249 6 View

Impact factor or works cited?

Which do you think is more important, impact factor or works cited, and why?

02 January 2018 926 9 View

What indicators applied for evaluating online catalogs at universities?

25 October 2016 9,989 3 View

How to prepare ceramic-polymer composite for thick film by solid state method?

eg.KNN-PVDF composite for thick film

09 June 2016 1,739 3 View

Which framework would you recommend for evaluating climate change public policy ?

Looking for some suggestions about a suitable framework for environmental public policy analysis. Basically, I want to identify to what extent the current adaptation responses are tackling the...

19 May 2016 9,933 9 View

How do I evaluate CD68 immunostaining for microglia activation?

I am working on immune reaction in Alzheimer's disease in mouse models at UCL. Currently I am doing co-immunostaining with antibodies against Iba1 (green) and CD68 (red). My question is: How to...

28 April 2016 3,622 6 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View