I am working with an AKTA Go equipment. And according to their manuals, it is not possible to use reverse flow in this type of equipment. However, to perform deeper or more effective cleaning in some types of columns, it is required to pass the cleaning reagents through the columns by reverse flow. For example, in Histrap HP, Capto Q and DEAE columns, among others. I thought about connecting the columns upside down, but.... Is it safe to connect such columns like that in those equipments? In the opposite direction than usual? Does anyone have some kind of bibliography or infographic to read about the subject and the procedures to do it?
In hplc we used to connect colums upside down in case of predicted column blockade to clean them opening the other end to waste if correct connector is found it can be connected but , need to be carefully check the column properties , before connecting
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