Etest was the most common methodology and GC agar base the most frequently used agar. In the last 5 years, there was a marked shift among participants to the use of EUCAST breakpoints from the CLSI breakpoints, most likely influenced by Euro-GASP and the publication of the EU case definitions in August 2012. However, the lack of a recommended methodology for Neisseria gonorrhoeae susceptibility testing by EUCAST might result in some laboratories continuing to use the CLSI breakpoints, which are inherently linked to the CLSI methodology, which may impact the interpretation.
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