I am supposed to look at a certain protein by FACS in naive and memory T cells from PBMCs of healthy blood donors. The naive-memory panel i used is:
CD3, CD4, CD8, CD45RA and CCR7
At 0h when the cells have not been activated or anything, this makes sense and i can see the different T cell subsets very well.
But I`m suppose to do the same thing after a 48h TCR activation as well (plated aCD3 + soluble aCD28). And in this situation, my naive-memory panel doesn`t make much sense for me.
Does anyone know a good reference for the change of the surface markers after TCR activation? Or does anyone have experience with this?
First... you can't have a "naive" memory T cell.
Or do you mean you are looking at naive and/or memory T cells?
And are you looking at central memory or effector memory T cells?
As for naive T cells, once activated, they become "effectors" and they tend to lose CCR7.
Without understanding what you are seeing with your data... I'm not sure how else to help.
As for reference, the best place now to look for this basic data is probably the vendors that sell T cell marker antibodies.
https://expertcytometry.com/differentiate-t-cell-state-with-flow-cytometry/
http://www.bdbiosciences.com/ds/pm/others/23-13010.pdf
Article Qualitative differences between na??ve and memory T cells
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