To determine spectrophometrically the production of conjugated linoleic acid using the protocol of Ribeiro et al. (Ribeiro, S. C., Stanton, C., Yang, B., Ross, R. P., & Silva, C. C. (2018). Conjugated linoleic acid production and probiotic assessment of Lactobacillus plantarum isolated from Pico cheese. LWT, 90, 403-411.) can samples be frozen before measuring at 233 nm with the spectrophotometer and measured at a later time?

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