Georgiana Toma

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Questions related from Georgiana Toma

Can I change the medium for a CFSE assay?

I need to test the effect of certain drugs on naive T cell proliferation. My problem is that for every other assay I do a 16h stimulation with the drugs. So I plate my cells with aCD3/aCD28 leave...

07 December 2018 6,138 2 View

Why do I get a 70% purity with CD4+ microbeads, but 97-99% purity with CD8+ microbeads?

Sometimes when I sort CD8+ cells first from PBMCs and then CD4+ cells, I get a very good purity with the CD8+ beads and a lousy purity with the CD4 beads. I try to keep the beads, buffer, even the...

05 October 2018 9,781 1 View

What happens to the surface markers of T cells after TCR activation?

I am supposed to look at a certain protein by FACS in naive and memory T cells from PBMCs of healthy blood donors. The naive-memory panel i used is: CD3, CD4, CD8, CD45RA and CCR7 At 0h when the...

27 April 2018 9,851 2 View