Hi, I got Caco-2 cells (p30) from cell bank, and cultured them with normal morphology until p33. But when it was p34, there were some different morphology cells. Normally, Caco-2 made big cell clones after 2~3days, but some round small cells around clones were not integrated and proliferated. I waited for 6~7days, but it was still same. I don't know why..
Culture media was DMEM high glucose with FBS 10% and anti-anti 1%.
Caco -2 cell properties change with culture conditions and passage number. One you have achieved the consistent cell behavior, stay within a relatively narrow range of passages, going back to lower end frozen stock when the upper end of your range is reached.
https://pubmed.ncbi.nlm.nih.gov/9083258/
Article Effect of Passage Number and Culture Time on the Expression ...
Hello
Your cells seem to be contaminated by other cells (the small ones who form white cloud). The best is to discard all.
Hello,
I found new ref.. I think It is because of EGF effect. Although I didn't treat anything to my cells, Cell morphology looked really similar with cells effected by EGF.
Here is ref.
Article Correlation between FAK and EGF-Induced EMT in Colorectal Cancer Cells
Thank you to answer my question.
Refer these pics.
First one is mine. It looks like EGF-induced EMT in Caco2.
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