What is the problem with my siRNA transfection?

I tried a pool of 3 siRNA transfection in Hela cells using RNAiMAX but all of of them failed. 100nM conc siRNA and opti-mem were used at 40~50% cell confluency and then 6 hour later, added DMEM...

02 September 2020 9,096 4 View

Why is methylene blue commonly used to measure an active surface area of nanoparticles?

Among the dyes, why is methylene blue commonly used to measure an active surface area of nanoparticles(especially graphene derivatives)? I try to find the reason, but I cannot find it. Could you...

11 March 2020 2,963 4 View

What is the meaning of this peak in small angle scattering 1D pattern?

In small angle neutron scattering SAS (actually small angle neutron scattering, SANS), I obtained a 1D pattern showing a peak which is located over 1 inverse angstrom. The actual peak position is...

23 December 2019 2,523 2 View

How can I increase the yield of this compound?

Hi guys, I want to synthesize the compound in the below picture. (Ref from Chem. Commun., 2017,53, 3189-3192 ) Its synthetic procedure seems gabriel synthesis using...

06 March 2018 6,385 3 View

Why does DMSO show quintet in 1H NMR?

Hi guys, I learned that dmso peak in 1H NMR caused by proton exchange. So the peak at 2.5 ppm indicates dmso-d5 not d6 Also the dmso which have more exchanged proton like d4 or others can be shown...

04 March 2018 8,655 3 View

Why brine(saturated NaCl solution) is better than water in extratction?

Hi, I've learned extraction using brine. The reason that I learned was increasing polarity and removing water in organic phase. Then, how this can happen and why brine is better than pure...

10 February 2018 5,516 3 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

Can I arbitrarily take a cycle number of 40 for the gene that was not expressed at all in qPCR results calculation?

I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...

20 February 2021 6,243 10 View

Do you know this error? The experiment was stopped by a potential overload on Vs (auxiliary electrode)?

Hello everyone Do you have any information about this error 'The experiment was stopped by a potential overload on Vs (auxiliary electrode)' in Origamaster software? I tried everything possible,...

19 February 2021 3,152 7 View

Is there a paper that shows the effect of the thermal conductivity of solid electrolytes on lithium secondary batteries?

I want to know the effect of thermal conductivity characteristics of solid electrolytes on the all-solid battery, but I don't know which paper to refer to. Is there a paper on thermal behavior?

15 February 2021 8,806 2 View

Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

Are there any exception like work hardening in metals elastic limit due to dynamic loading?

The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...

11 February 2021 952 6 View

How to avoid polypyrrole deposit on counter electrode?

Hi all, Currently, I electrodeposited polypyrrole on carbon electrode but I got some problem when polypyrrole also deposited on the surface of the counter electrode. The concentration of pyrrole...

05 February 2021 2,103 3 View

Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View

Queries regarding the rain flow counting algorithm?

02 February 2021 9,790 2 View