We just added a custom heated microscope stage to our inverted microscope....but have found out that all our cell flasks and multi-well plates have tiny ('stacking friendly') perimeter ridges that protrude about 1 mm out the bottom and that prevent the heated stage from making direct contact with the body of the flask....so we can't get the cell media up to 37 deg C inside the flasks or 12 well plates. Where can we get flasks/plates that are 'non stacking' and don't have these little ridges that keep the cells off of the heated stage plate?
Here are two close up photos showing our problem.... the cell culture flask has tiny ridges on the bottom that prevent the flask from being in direct contacted with the heated microscope stage. This prevents the cell media from reaching 37 Degrees C because of the small (1mm) air gap between the bottom of the cell flask and the heated microscope stage plate (round silver plate in photos). Perhaps somebody knows of some cell flasks which do not have these small ('stacking') ridges on the bottom?
This ones have uniform ridges http://www.tpp.ch/page/produkte/01_zellkultur_flasche.php
other option without ridges, but im not sure plase check: http://www.merckmillipore.com/ES/es/life-science-research/cell-culture-systems/cell-growth/hy-flask/2Vyb.qB.c84AAAFBbK5b3.rc,nav?cid=BI-XX-BSP-P-GOOG-CELL-B344-1023
i hope it can help you
Did you have solved that problem?
Pd: could we have a videoconference? Maybe i could help you with your foundraising
Hello, We solved the problem by making a small aluminum plate ('shim') that fits under the T25 flask and fills the air gap....so now the flask heats properly from the heated microscope plate. If you wish to contact me directly, please email me at: [email protected] Thank you!
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