Anthony Holland

6 Questions 10 Answers 0 Followers

Questions related from Anthony Holland

Need Help calculating % FBS in final solution?

Can somebody please help me confirm the final percent of FBS in the following mixture: 500mL stock media, add 100ml FBS (FBS concentration is now 16.66%, right?). Now remove 12mL of that mix and...

05 November 2016 4,036 3 View

What flasks/12 well plates to use with heated microscope stage?

We just added a custom heated microscope stage to our inverted microscope....but have found out that all our cell flasks and multi-well plates have tiny ('stacking friendly') perimeter ridges that...

06 July 2016 1,761 6 View

How many trypan blue assays do I need to have meaningful data?

We are doing cancer cell experiments in vitro. We use the trypan blue assay method for viable vs. nonviable cells. If we are 'treating' a 12 well plate or a T25 flask, how many assays do we need...

30 June 2016 1,668 3 View

Could the electric fields be changing the PH of A549 cell culture media making the phenol red more red?

We are treating A549 cells with pulsed electric fields. The media becomes more redish after prolonged treatments (11 hours). When the cells are returned to the incubator overnight, the PH...

03 June 2015 2,831 5 View

K562 cells, can you just add media to flask?

If you are growing K562 cell line in a culture flask (25 cm2), can you just add some media every 2 or 3 days to keep the cells 'happy', or do you need to always centrifuge the cells and remove the...

15 January 2014 6,086 1 View

How often to assay cell culture experiments

Dear colleagues, how often should a cell culture be assayed during a 3 day experiment? Once at the start and then at the end or every 12 hours? We worry that every 12 hours is too stressful for...

01 January 1970 4,001 4 View