Anthony Holland

4 Questions 4 Answers 0 Followers

Questions related from Anthony Holland

How many trypan blue assays do I need to have meaningful data?

We are doing cancer cell experiments in vitro. We use the trypan blue assay method for viable vs. nonviable cells. If we are 'treating' a 12 well plate or a T25 flask, how many assays do we need...

30 June 2016 1,586 3 View

Could the electric fields be changing the PH of A549 cell culture media making the phenol red more red?

We are treating A549 cells with pulsed electric fields. The media becomes more redish after prolonged treatments (11 hours). When the cells are returned to the incubator overnight, the PH...

03 June 2015 2,766 5 View

K562 cells, can you just add media to flask?

If you are growing K562 cell line in a culture flask (25 cm2), can you just add some media every 2 or 3 days to keep the cells 'happy', or do you need to always centrifuge the cells and remove the...

15 January 2014 5,998 1 View

How often to assay cell culture experiments

Dear colleagues, how often should a cell culture be assayed during a 3 day experiment? Once at the start and then at the end or every 12 hours? We worry that every 12 hours is too stressful for...

01 January 1970 3,945 4 View