Which search engines can I use to get the source of a certain microbial strain?

I am searching for a certain website through which can I get the source of certain microbial strains, especially probiotic strains I mean a search engine contains a database of the identified...

20 December 2020 5,144 5 View

Can you help me choose a topic to my thesis?

Good Morning, I am a Ph.D. student studying " Information Management & Libraries" interested to work on a topic related to technology and how it is changing the future of libraries, but I...

14 December 2020 3,848 14 View

I need to know my H-index, my citations and publications?

How can I obtain my H-index, citations, publications?

01 December 2020 7,589 6 View

Why I am losing the Zona of pellocida while doing mouse preimplementation embryo staining?

I am doing some staining experiments in mice embryos and I noticed that I lose the zona of pellocida during the procedures. I can not define at exactly which step I lose it but mostly either...

15 October 2020 2,906 2 View

How to prepare 0.2 M Sodium Acetate Buffer Solution, pH 3.5?

12 October 2020 9,558 3 View

How to preserve Fe3O4@SiO2@Chitosan nanoparticles for a long time?

Is freeze-drying an appropriate method for these types of nanoparticles? If it's ok, what are the relevent buffers and conditions?

11 October 2020 2,276 3 View

Do you know of any source to buy hemp seeds?

Industrial hemp seeds with a good germination rate

28 September 2020 7,568 3 View

Is there a Deep Learning baseline (preferably written in PyTorch) for predicting animal pose from scratch (not like DeepLabCut or DeepPoseKit)?

I am looking for a animal pose estimation code that predicts the pose of an animal given 100-200 annotated frames from scratch using deep learning on a frame-by-frame basis. Is there any such...

27 September 2020 8,957 3 View

How to get Databases for MENA region banks?

I need to collect databases for Banks in the MENA region for my study, is there any public database or other databases that are affordable to access? Thank you.

30 August 2020 5,669 9 View

What is information system, data mining journal to publish survey/literature review paper ?

Your suggestion for an information system, data mining journal for publishing survey/literature review paper

13 March 2020 4,720 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

What statistical test is required for an analysis on pre-COVID & post-COVID patient improvement after attending a 10-week programme?

Hi all, Myself and my supervisor are looking at carrying out a statistical analysis to see whether there is a difference in patient outcomes for our 10-week pain management programme according to...

10 February 2021 6,622 10 View

How do you prepare a standard dilution for qPCR?

I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...

05 February 2021 9,834 4 View