What selection markers to use for the generation of stable HEK293 cell line ?

Dear all, I am trying make stable clones of a HEK293 using an antibiotic selection strategy. However, the parental cell line is already resistant to the five commonly used antibiotics (G418,...

14 August 2020 7,542 2 View

What are your approaches and practices regarding games in the EFL classroom?

Dear fellow EFL teachers, we, Laura Schmetzer and Lennart Holzhuetter, are currently writing our master's thesis on the topic of games in the EFL classroom and would really appreciate it, if you...

28 July 2020 3,077 2 View

How can I export data (csv) from scopus effecient to an excel table?

Hey community i have a question for you. Im doing a literature review and i want to put the relevant literature in an excel table to check it later. Whenever i am exporting something in CSV (using...

13 January 2020 5,916 3 View

How does Burying beetle Nicrophorus vespilloides burying and copulating act look like?

I have read several papers about Nicrophorus vespilloides burying and copulation and I still have some questions. -Do only males bury the carrion? -Does male figth with intruders or also female?...

11 February 2018 8,481 9 View

Can you identify those bird feathers?

19 April 2017 2,715 3 View

Do I need permission to collect invertebrates from Gran Canaria?

I am going to Gran Canaria and planning to collect some invertebrates. Do I need permission for that and what should I do for getting the permission? I will collect for natural history museum of...

14 January 2017 5,777 15 View

Would you please identify those freshwater aquarium species?

20 October 2016 5,370 7 View

Can you identify those fish?

Please help me identify those fish. My oppinion 1-3 Taurulus bubalis 4-5 Cyclopterus lumpus

16 June 2016 725 6 View

What species of Mollusca are these specimens?

That species of Mollusca was found from lake Viljandi (https://www.google.ee/maps/@58.3483153,25.6106634,14z?hl=en). This summer. The measurements of shell are shell height 4,5mm shell width...

09 December 2014 2,540 12 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

Where would? can? positive charge(vacancy) be when grounded?

Hello I plasma(H2N2) treated Al2O3 plate (edge is connected to ground) and wondered if oxygen vacancy migration on the plate can occurs as electrons escape through edge. Or whatever positive...

15 February 2021 8,118 1 View