try with STE biffer SDS Tris Cl and EDTA buffer

STE buffer is composed of 100 mM NaCl, 1 mM EDTA, 10 mM Tris/HCl pH 8.0

To prepare one liter of this buffer you have to weigh in:

5.84 g NaCl (molar mass 58.44 g/mol)

1.21 g Tris (molar mass 121.14 g/mol)

0.29 g EDTA (molar mass 292.24 g/mol)

fill up with distilled water to ~900 ml

adjust the pH with HCl to 8.0

fill up with distilled water exactly to 1 liter

Thank you Phani Kashyap S. 

Phani Kashyap S. 

How many percentage of SDS should be added to make STE buffer...?