I am about to finish a manuscript about High-throughput screening of biocatalysts from metagenomic DNA library, I am in search of a Q1 journal with no limited words, pages or figures where I can publish my work.
Any suggestion?
While using 3 fluorogenic substrates derived from Coumarins in cellulose- and xylan-degrading enzymes screening, one of them (MUGlc) shows blue background fluorescence during enzyme assay. is...
31 December 2018 421 3 View
For my recombinant enzymes, I am not able to see any peak in the elution side of the FPLC results using his-tag column, while a peak is present before elution. is anyone able to interpret what...
07 August 2018 9,224 2 View
What are parameters that decide the novelty of an enzyme before characterize it? means If we have a protein sequence which shows certain similarity to a protein in the database, what percentage...
01 February 2018 5,719 11 View
I tried to do multiplex colony PCR, I pooled many clones into one tube and I run colony PCR and I did not get bands. To make sure that the problem is not my primers' design, I have extracted the...
31 December 2017 9,074 18 View
How can we interpret SASA results when compare between Enzyme and Enzyme-ligand complex?
31 December 2017 2,544 0 View
I have faced this with more than one gene. in the step of gene annotation of my NGS data I found in many case my enzyme of interest but I found that this enzyme is found in multispecies, it means...
11 December 2017 9,593 1 View
After proceeding with Prodigal for Genes prediction we would able to blast it and find known genes in our data. Is it possible to find novel genes by comparing it to conserved domain database CDD?...
11 December 2017 4,004 7 View
Is there any manual way to isolate recombinant fosmid DNA from E.coli cells in the absence of isolation Kit?
03 April 2017 3,765 4 View
I am planning to amplify a large DNA sequence of 40kb with PCR. Can I use a normal Taq Polymerase? Is there any specific one for large sequences amplification?
11 December 2016 8,893 30 View
For how many time can we store bacteria in glycerol stock in -80 degree? Do we have to transfer the bacteria to fresh media with glycerol after an approximate duration? In addition to Freeze-Thaw...
11 December 2016 7,466 22 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.
06 August 2024 6,796 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
As authors and academic writers, we usually prepare our manuscript using our own resources. We submit these manuscripts to scientific Journals for peer review and publication sometimes at a fee....
03 August 2024 7,304 2 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...
30 July 2024 435 3 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View
Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...
28 July 2024 6,553 1 View