How can we interpret SASA results when compare between Enzyme and Enzyme-ligand complex?
While using 3 fluorogenic substrates derived from Coumarins in cellulose- and xylan-degrading enzymes screening, one of them (MUGlc) shows blue background fluorescence during enzyme assay. is...
31 December 2018 421 3 View
For my recombinant enzymes, I am not able to see any peak in the elution side of the FPLC results using his-tag column, while a peak is present before elution. is anyone able to interpret what...
07 August 2018 9,224 2 View
I am about to finish a manuscript about High-throughput screening of biocatalysts from metagenomic DNA library, I am in search of a Q1 journal with no limited words, pages or figures where I can...
03 April 2018 1,870 0 View
What are parameters that decide the novelty of an enzyme before characterize it? means If we have a protein sequence which shows certain similarity to a protein in the database, what percentage...
01 February 2018 5,719 11 View
I tried to do multiplex colony PCR, I pooled many clones into one tube and I run colony PCR and I did not get bands. To make sure that the problem is not my primers' design, I have extracted the...
31 December 2017 9,074 18 View
I have faced this with more than one gene. in the step of gene annotation of my NGS data I found in many case my enzyme of interest but I found that this enzyme is found in multispecies, it means...
11 December 2017 9,593 1 View
After proceeding with Prodigal for Genes prediction we would able to blast it and find known genes in our data. Is it possible to find novel genes by comparing it to conserved domain database CDD?...
11 December 2017 4,004 7 View
Is there any manual way to isolate recombinant fosmid DNA from E.coli cells in the absence of isolation Kit?
03 April 2017 3,765 4 View
I am planning to amplify a large DNA sequence of 40kb with PCR. Can I use a normal Taq Polymerase? Is there any specific one for large sequences amplification?
11 December 2016 8,893 30 View
For how many time can we store bacteria in glycerol stock in -80 degree? Do we have to transfer the bacteria to fresh media with glycerol after an approximate duration? In addition to Freeze-Thaw...
11 December 2016 7,466 22 View
i am unable to interpret why its increases in start as shown in figure
11 August 2024 2,179 1 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
How can I interpret the data gathered without solving?
03 August 2024 9,054 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...
01 August 2024 5,460 5 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?
26 July 2024 4,741 1 View
While doing AST for Pseudomonas aeruginosa, after incubation, no zone of inhibition observed in the plate near the well. wells surrounded by bacterial growth, when the same plate observed under UV...
25 July 2024 9,229 1 View
Hi, I am currently a upcoming 4th year student who is need of your help as I couldn't find any accessible file for the manual scoring and interpretation for the PBOI - White Campbell. My group and...
23 July 2024 2,269 1 View