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Questions related from Farah Fadwa Ben belgacem
While using 3 fluorogenic substrates derived from Coumarins in cellulose- and xylan-degrading enzymes screening, one of them (MUGlc) shows blue background fluorescence during enzyme assay. is...
01 January 2019 385 3 View
For my recombinant enzymes, I am not able to see any peak in the elution side of the FPLC results using his-tag column, while a peak is present before elution. is anyone able to interpret what...
08 August 2018 9,187 2 View
I am about to finish a manuscript about High-throughput screening of biocatalysts from metagenomic DNA library, I am in search of a Q1 journal with no limited words, pages or figures where I can...
04 April 2018 1,822 0 View
What are parameters that decide the novelty of an enzyme before characterize it? means If we have a protein sequence which shows certain similarity to a protein in the database, what percentage...
02 February 2018 5,670 11 View
How can we interpret SASA results when compare between Enzyme and Enzyme-ligand complex?
01 January 2018 2,508 0 View
I tried to do multiplex colony PCR, I pooled many clones into one tube and I run colony PCR and I did not get bands. To make sure that the problem is not my primers' design, I have extracted the...
01 January 2018 9,020 18 View
After proceeding with Prodigal for Genes prediction we would able to blast it and find known genes in our data. Is it possible to find novel genes by comparing it to conserved domain database CDD?...
12 December 2017 3,969 7 View
I have faced this with more than one gene. in the step of gene annotation of my NGS data I found in many case my enzyme of interest but I found that this enzyme is found in multispecies, it means...
12 December 2017 9,553 1 View
Is there any manual way to isolate recombinant fosmid DNA from E.coli cells in the absence of isolation Kit?
04 April 2017 3,717 4 View
I am planning to amplify a large DNA sequence of 40kb with PCR. Can I use a normal Taq Polymerase? Is there any specific one for large sequences amplification?
12 December 2016 8,791 30 View
For how many time can we store bacteria in glycerol stock in -80 degree? Do we have to transfer the bacteria to fresh media with glycerol after an approximate duration? In addition to Freeze-Thaw...
12 December 2016 7,425 22 View
In my assay microtiter screening I am doing fluorescence measurement using Tecan microplate reader, I found that it is recommended to use the optimal gain function for application that produce...
08 August 2016 5,348 7 View
Dear all We have prepared metagenomic DNA library and now we are screening this library following the high throughput screening method and using fluorogenic substrates to find Hydrolytic enzymes....
02 February 2016 7,445 3 View
Dear all Can any one suggest some statistical software used to analyse screens of high throughput screening and hopefully it is applicable for our case (looking for hydrolytic enzymes using...
02 February 2016 4,896 4 View
I am doing High Throughput Screening HTS of functional metagenomic library to screen for cellulose-degrading enzymes using fluorogenic substrate, I want to know what is the best lysis solution for...
01 January 2016 812 3 View
I am about to perform metagenomic DNA extraction, and one of the extraction buffer composition is CTAB (Cetyl Trimethyl Ammonium Bromide), unfortunately we don't have CTAB here.
01 January 2016 8,669 3 View
