What are parameters that decide the novelty of an enzyme before characterize it? means If we have a protein sequence which shows certain similarity to a protein in the database, what percentage this enzyme needs to be novel????
I don't think there are rules for this. In the beta-lactamase field, a single amino acid change is sufficient for the enzyme to receive distinction as a new enzyme within an existing class, referenced by a number, such as KPC-2, KPC-3, etc. There are thousands of individually named beta-lactamases as a result.
I think it also depends on whether they are orthologues or paralogues and how different the catalyzed reactions are. But really do not expect a single number.
The novelty of an enzyme is largely dependent on many factors and properties on the said enzyme. The novelty could be in its source, physico-chemical, kinetics, structural or functional properties and in this case, a little difference as low as one in the amino acid sequence. As such, there is no rigid rule in determining if the enzyme is novel. However, if the characterized property is very similar to that of a known enzyme, the degree of its novelty is subjective to the reviewer.
Novelty can be due to
1. lack of previous characterization of the enzyme despite its annotated gene information or not.
2. synthesis (de novo design of protein) with new functional properties: stability, efficiency/productivity, specificity; for existing or new reaction.
Novelty is the same in science as in art!!! - Toluwase H. Fatoki
Dear Adam B Shapiro,
Always I have to thank you for your answers on my questions.
Is there any paper which defends this (to be included in my manuscripts)?
Thank you Tomáš Hluska
For the moment I do not have yet idea about this, I want to know in the DNA and Protein sequences step whether this enzyme is new or not.
Cordially!
Dear Toluwase H. Fatoki,
Thanks a lot, any papers for more understanding that you may suggest please?!
Regards!
Concerning beta-lactamase nomenclature:
http://aac.asm.org/content/50/4/1123.full
Article Exploring the Evolution of Novel Enzyme Functions within Str...
Article Advances in Enzymology: And Related Areas of Molecular Biolo...
Article Tools and strategies for discovering novel enzymes and metab...
https://www.nwo.nl/en/research-and-results/research-projects/i/02/15502.html
https://novelenzymes2018.eu/
Thank you so much Toluwase H. Fatoki , So nice of you.
Regards!
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