What is the mol. weight of GFP-11 tag?

Hi, I am trying to find out the mol. weight of GFP 11 because it is tagged to my protein of interest which is 17kDa. GFP 11 has been tagged with my protein of interest while GFP 1-10 is tagged...

09 December 2020 1,863 1 View

How should I store the milk sample in RNAlater?

I want to extract RNA from human breast milk. How should I store the milk sample in RNAlater? And after RNA extraction how can I store the RNA?

01 December 2020 7,183 4 View

Which is the best stripping method for proteins having molecular weight 90kDa and above?

Hi, I would like to know which is the best stripping method for proteins having mol. wt. around 90 kDa or more. I am using human samples. I used 0.5M EDTA stripping buffer by boiling PVDF...

08 September 2020 5,815 2 View

What is the best solvent for dexamethasone so that it does not get toxic for the cells?

Hi, I am using C2C12 cells. I need to treat them with dexamethasone. I would like to know which is the best solvent to dissolve dexamethasone so that it is not toxic to the cells? I am using dex...

22 June 2020 4,870 6 View

How to build alignment files for homomeric and heteromeric proteins using Modeller?

I am building protein structures for a list of query proteins via homology modelling using Modeller. In some cases the template is a homomeric protein and in others its a heteromeric protein....

01 April 2020 6,576 3 View

How to correctly standardise migration assay using HEK 293T cells, without the use of matrigel in transwell?

So, I have been trying to quantify the migrated cells through transwells without using Matrigel (because I just want to quantify the natural migration rate of HEK 293T). 1. I plate them on the...

19 February 2020 7,123 1 View

How to measure vagal tone index?

07 October 2019 9,748 3 View

How to simulate water Platinum interaction in LAMMPS?

I am working on water drop simulation on Platinum surface using LAMMPS. EAM is used for Pt and TIP4P for water, but how to model the interaction between water and Platinum using LAMMPS?

06 June 2016 6,340 2 View

ER stress induction in Hap1 cells?

26 April 2016 7,145 3 View

Does anyone have experience with transfection gRNA and cas9 mRNA using RNAimax?

Does anyone have experience with transfection gRNA and cas9 mRNA using RNAimax? What would I select the transfection positive cells with, given that I'm transfecting RNA?

25 February 2016 1,865 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View